42例脑星形胶质细胞瘤P53蛋白表达及与PCNA的关系

Expression of P53 protein in 42 human astrocytic tumors and its relation with expression of PCNA

目的：观察人脑星形胶质细胞瘤中Ｐ５３蛋白的表达及与增殖细胞核抗原（ＰＣＮＡ）表达的关系，以深入探讨Ｐ５３蛋白的表达在脑星形胶质细胞瘤发生中的作用。方法：用Ｐ５３蛋白抗体ＰＡｂ１８０１和ＰＣＮＡ的抗体Ｐｃ１０检测４２例脑星形胶质细胞瘤切除标本，以阳性指数的表达方式，对两种免疫组化的结果进行定量，并对其中资料完全的３５例Ｐ５３蛋白和ＰＣＮＡ的阳性指数进行相关性分析。结果：在各级星形胶质细胞瘤中均有Ｐ５３阳性表达；Ｐ５３阳性指数分别为Ⅰ级１４．６６±１３．９７，Ⅱ级３６．５４±１９．２４，Ⅲ～Ⅳ级２８．８７±２５．２５；Ⅰ级和Ⅱ级组有显著差异（Ｐ＜０．０５）。Ｐ５３和ＰＣＮＡ两者的阳性指数呈非常显著的正相关（ｒ＝０．５１２）。结论：提示Ｐ５３蛋白在调控细胞增殖、促成脑星形胶质细胞瘤的生成中具有一定作用。

Objective:To clarify the correlation between the expression of P53 protein and that of PCNA,then to define further the effect of P53 expression on tumorigenesis of astrocytic tumors.Methods:42 surgical specimens of human astrocytic tumors were immunostained with a monoclonal antibody against P53 protein(PAb1801),and a monoclonal antibody against PCNA(Pc10).By counting positive index(PI),we quantitated the results of immunohistochemistry and compared the correlation between PI of P53 protein and PCNA. Results:P53 expression was observed in the tumors of all grades.The PI of P53 protein was 14.66±13.97 in grade Ⅰ, 36.54±19.24 in grade Ⅱ and 28.87±25.25 in grade Ⅲ ￣ Ⅳ,respectively.There were significant differences in PI between grade Ⅱ and Ⅰ.We found that expression of P53 protein was related to that of PCNA,and there was a positive correlation between PI of P53 and PCNA.Conclusion:P53 palys a role in modulating cell proliferation and promoting astroglioma tumorigenesis.