小剂量IL－2促进大鼠脑损伤后记忆功能恢复

Effects of rhIL-2 on memory after moderate fluid percussion of brain injury in rats

目的：为探讨白细胞介素２（ＩＬ－２）对脑损伤后记忆功能恢复的作用机理。方法：采用测方液压大鼠脑损伤模型，观察侧脑室注射（ｉｃｖ）ｒｈＩＬ－２对大鼠记忆功能和组织病理变化的影响。实验选用雄性ＳＤ大鼠，伤后１～３ｄ连续ｉｃｖｒｈＩＬ－２，分３组（３０，６０，３００Ｕ），ＮＳ治疗为对照组。水迷宫试验测定大鼠记忆功能。结果：发现ｒｈＩＬ－２能明显改善大鼠脑损伤后学习记忆功能，尤以６０Ｕ治疗效果最为显著；并且能缩小皮层空洞，６０Ｕ组伤后２周时的空洞面积为０．０７ｍｍ×０．１０ｍｍ，ＮＳ组为０．５１ｍｍ×１．０ｍｍ，相差显著（Ｐ＜０．０１）；ｒｈＩＬ－２治疗组海马ＣＡ２，ＣＡ３区神经元死亡较少。结论：小剂量ＩＬ－２对神经元具有显著保护作用和促进创伤修复作用。

Objective: To evaluate the effect of recombined human interleukin-2(rhIL-2) on memory ability and histopathologic changes. Methods : The rhIL-2 was administrated by intracerebroventricular (icv) injection in 3 d after injury with 30, 60 and 300 U in each group whereas injection of NS served as control. Results:The rhIL-2 could improve markedly the memory deficits of rats after brain injury, especially at 60 U treatment. The cortical cavity with 60 U rhIL-2 injection was smaller than NS injection, 0. 07 mm× 0. 10 mm vs 0.sl mm×1. 0 min (P<0. 01). And we also observed that rhIL-2 could support the survival of the neurons in the hippocampal CA2 and CA3 regions. Conclusion: Our data indicate that rhIL-2 may have the protective function for CNS neurons in vivo, and activate wound repair.