海风藤醇B对PAF越膜代谢和生物合成的影响

Effects of Piperol B on transmembrane metabolism and biosynthesis of PAF in rabbit platelets

目的：研究海风藤醇Ｂ的抗血小板激活作用与其影响兔血小板中血小板激活因子（ＰＡＦ）的越膜代谢和生物合成间的关系。方法：采用放射性薄层层析法和ＰＡＦ生物测定法研究海风藤醇Ｂ对ＰＡＦ越膜代谢和生物合成的影响。结果：海风藤醇Ｂ、银杏内酯Ｂ（ＢＮ５２０２１）可抑制兔血小板［３Ｈ］－ＰＡＦ的越膜代谢，ＩＣ５０分别为８４．８，３４．９μｍｏｌ·Ｌ－１。海风藤醇Ｂ（１０，５０μｍｏｌ·Ｌ－１）对Ａ２３１８７刺激的兔血小板生成ＰＡＦ无抑制作用，高浓度（１００μｍｏｌ·Ｌ－１有轻微抑制，抑制率为１６．３％。结论：海风藤醇Ｂ可能通过对兔血小板ＰＡＦ的越膜代谢的抑制而影响血小板激活。

To look into the relationship between anti-activating of rabbit platelets of Piperol B and its effect on the transmembrane metabolism and biosynthesis of platelet-activating factor(PAF) in rabbit platelets. Methods: Using radioactive thin-layer chromatography and bioassay of PAF, we studied the effect of Piperol B on the transmembrane metabolism and biosynthesis of PAF in rabbit platelets. Results: Piperol B and Ginkgolide B(BN52021) could inhibit the metabolism of [3H]-PAF in rabbit platelets, IC50s were 84.8 , 34.9μmol· L-1, respectively. Piperol B(10, 50μmol·L-1) had no effect on the biosynthesis of PAF stimulated by A23187. High concentration of Piperol B (100μmol·L-1)had low inhibition of PAF production, and the inhibition was 16.3 %. Conclusion: The anti-activating effect of Piperol B was attributed to its inhibition of Piperol B of transmetabolism of PAF.