静脉注射用胰岛素三嵌段共聚物纳米粒的制备

Preparation of insulin-loaded triblock copolymeric nanoparticle drug delivery system for intravenous injection

目的研究静脉注射用胰岛素三嵌段共聚物纳米粒（Ins-PELGE-NPs）的制备工艺，并考察其影响因素。方法通过复SL／溶剂扩散法制备Ins-PELGE-NPs，并考察其形态、粒径分布、包封率、zeta电位等。结果载药量为4．48％～4．67％的Ins-PELGE-NPs在透射电镜下均呈球形或近球形，分布均匀，平均粒径为140nm，平均包封率为94％～98％，平均zeta电位为-21．7±4．3mV。结论所用制备工艺简单、包封率高，可用于系列注射用三嵌段共聚物纳米粒的制备。

OBJECTIVE To study the preparation of insulin - loaded triblock copolymeric nanoparticles （ Ins - PELGE - NPs） and their influence factors on the characteristics of Ins - PELGE - NPs. METHODS The Ins - PELGE - NPs were prepared using a water - in - oil - in - water （ W1/O/W2 ） emulsion solvent diffusion technique. The characteristics of the nanoparticles were studied in terms of morphology, particle size distribution, encapsulation efficiency （EE） and zeta potential. RESULTS The Ins - PELGE - NPs with insulin loading of 4.48% -4.67% in transmission electron microscopy （TEM） was mostly spherical in shape. The average size of NPs was about 140 nm with EE ranged in 94% -98% and zeta potential of -21.7 ± 4.3 mV. CONCLUSION Optimized preparation parameters led to nanoparticles with well - defined characteristics such as high EE and might be used to prepare a series of drug loaded triblock copolymeric nanoparticles for drug delivery system of intravenous injection.