高原缺氧环境成年男性精子的计算机辅助分析

A computer-assisted semen analysis of mature males living in hypoxia environment at high altitude

目的：了解长期缺氧暴露对成年男性精子功能参数的影响。方法：以28名留驻海拔5300m1-3年的健康成年男性青年为高原组，以生活在海拔1300m的31名同龄成年男性为对照组。常规收集精液标本，采用计算机辅助精液分析（CASA）系统对精子功能进行实时动态参数分析。结果：高原组的精子密度明显低于对照组（P〈0．05），精子活率低于对照组但未见显著差异。高原组精子运动的轨迹速度（VCL）、直线速度（VSL）、角速度（VAP）和直线运动率（LIN）参数均存在明显下降的趋势（P〈0．01）。结论：长期驻留高原缺氧环境对成年男性的精子功能有负面影响。

Objective： To explore the influence of long - term hypoxia exposure on the function of sperm of mature males. Methods：A total of 28 adult males stayed at altitude of 5 300 m for 1 to 3 years were recruited as highaltitude group and 31 age -matched young men living at altitude of 1 300 m were served as control group. Semen specimens were collected by routine method, and real - time semen analysis was performed by using computerassisted semen analysis （CASA） system. Results： The sperm density in high - altitude group was significantly lower than that in control group （P 〈 0.05 ）. The ratio of living sperm in high - altitude group was lower than that in control group, without significant difference. There were obviously decreasing tendencies in curvilinear velocity （ VCL）, straight - line velocity （ VSL）, average path velocity （VAP） and linearity （ LIN ） compared with control group （P 〈0.01 ）. Conclusion：Long -term staying at hypoxia high altitude has multiple negative effects on sperm function of mature males.