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猪CD86 mRNA定量检测方法的建立

Development of Quantitative Detection of Porcine CD86 mRNA
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摘要 用RT-PCR技术从猪肺泡巨噬细胞中扩增和克隆了猪CD86基因部分片段,经测序鉴定后,用反向PCR技术构建了CD86的缺失竞争cDNA分子。通过竞争PCR方法,建立了CD86标准竞争曲线,并得到其直线回归方程。本方法操作简单,特异性和敏感性高,可用于猪CD86 mRNA水平的定量检测。 Porcine CD86 was amplified by RT-PCR from porcine alveolar macrophages and cloned. Following sequencing, competitive depletion clones for the cDNA molecules was generated by reverse PCR. Then, the linear regression equation was obtained after construction of the standard competitive curves by quantitative competitive PCR assay. This study provided a simple, high specific and sensitive method for defecting porcine CD86 mRNA level.
作者 周双海 杨汉春 郭鑫 陈艳红 查振林
机构地区 中国农业大学动物医学院
出处 《北京农学院学报》 2006年第2期33-35,共3页 Journal of Beijing University of Agriculture
基金 教育部跨世纪优秀人才基金(2003)资助项目
关键词 猪 CD86 竞争PCR porcine CD86 competitive PCR
分类号 S852.4 [农业科学—基础兽医学]
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参考文献7

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共引文献15

北京农学院学报
2006年 第2期

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