IFN-γ在怀孕早期山羊下丘脑-垂体-性腺轴的表达

Expression of IFN-γ immunoreactivity on HPG axis of dairy goat during early pregnancy

为了探讨γ-干扰素(in terferon-,γIFN-γ)在下丘脑-垂体-性腺轴(hypotha lam ic-p itu itary-gonada lax is,HPG轴)中的免疫调节作用,应用免疫组织化学SP法对IFN-γ免疫反应阳性产物在怀孕早期山羊HPG轴的分布特点进行了研究。结果显示,IFN-γ分布于乳头体内侧核、视前内侧核、下丘脑前区、腹内侧核、连接核、室旁核、视上核等20个核团中,各部分核团细胞着色程度深浅不一;在视上核、室旁核分泌部、乳头体内侧核等有大量的阳性神经纤维;同时在室周核、视前外侧核、视前内侧核等13个核团中散布有一些阳性星形胶质细胞,与阳性神经细胞交错共存;腺垂体中腺细胞和神经垂体中神经纤维均有IFN-γ表达;卵泡和黄体中也有IFN-γ存在。表明IFN-γ与动物的神经内分泌活动关系密切,参与了免疫-神经-内分泌网络中的生殖免疫调节。

To probe into the immunomodulation of IFN-γ to hypothalamic-pituitary-gonadal axis, the distribution of IFN-γ immunoreactivity on HPG axis of dairy goat during early pregnancy was determined by SP method of immuno-histochemistry. The result showed that IFN-γ was distributed over 20 nucleus of the hypothalamus,such as nucleus mamillaris medialis,nucleus pre-opticus medialis,area anterior hypothalami, nucleus ventromedialis, nucleus reunions, nucleus paraventricularis, nucleus supra-optic, and etc. Many of IFN-γ positive nerve fibers were found in supra-optic nucleus,paraventricular nucleus, nucleus mamillaris medialis and etc. Nerve fibers and astrocytes IFN-γ immunoreactive positive cells spreaded in periventricular nucleus, nucleus pre-opticus lateralis, nucleus pre-opticus medialis and etc. At the same time, IFN-γ is also observed obviously in adenohypophysis and neurohypophysis. IFN-γ also expresses in follicular and corpus luteum. The results suggest that IFN-γ is related to the activity of neuroendocrine of animals,and is concerned with the reproductive immunity regulation in immuno-neuro-endocrine network.