纳米超顺磁性碘油肝动脉栓塞热疗VX2兔肝肿瘤的实验研究

Experimental study of treatment on rabbit liver VX2 tumor by selective hepatic artery embolization hyperthermia with nano superparamagnetic iodized oil

目的观察纳米超顺磁性碘油经肝动脉栓塞热疗对实验性肝肿瘤的疗效。方法VX2兔肝癌模型24只,分成纳米超顺磁性碘油栓塞热疗组(A组)、碘油栓塞组(B组)、纳米超顺磁性碘油栓塞组(C组)、对照组(D组),每组6只。VX2肝肿瘤模型接种后14 d进行治疗,3个治疗组VX2兔均采用3F SP微导管经右股动脉插管,选择性进入肝固有动脉,注射10%的纳米超顺磁性碘油0．5 ml(A组和C组)或超液化碘油0．5 ml(B组)。注射后3 d,A组和B组动物置于裂隙式交变磁场中热疗30 min。而C组和D组动物不进行磁诱导热疗。各组兔于治疗前CT检查,测量肿瘤大小,栓塞后14 d再次作CT检查,检查结束后处死,取肝脏、脾脏、肾脏、心肌、肺组织作病理组织学检查,并直接测量兔肝脏肿瘤大小。结果所有兔均存活至处死,生化检查显示各组栓塞前和栓塞后14 d的肝肾功能无明显变化。治疗后14 d,纳米超顺磁性碘油栓塞热疗组兔肝肿瘤平均缩小了8．09%,碘化油栓塞组和纳米超顺磁性碘油栓塞组肿瘤分别增长了9．72%和13．00%,A组分别与B、C组对比,差异有统计学意义(P＜0．05)。而对照组肿瘤增长了57．50%,与其他组对比,差异有统计学意义(P＜0．01)。病理组织学检查,各治疗组肿瘤均有明显的坏死,肿瘤实质内几乎无肿瘤细胞残留,其中栓塞热疗组最明显。结论动物实验表明,纳米超顺磁性碘油栓塞热疗具有明显的抑制肿瘤生长的效果,有必要对其进一步研究开发,使得这一技术早日应用于临床。

Objective To observe the therapeutic response of liver tumors by arterial embolization hyperthermia with Nano Superparamagnetic Iodized Oil （NSIO） using rabbit VX2 liver tumor model. Methods A total 24 rabbits containing experimental hepatic tumors were randomly assigned to one of four groups as follows： NSIO embolization hyperthermia group（ group A）, Lipidol embolization group（ group B）, NSIO embolization group （group C）, and contronl group（ group D）, each groups contain 6 VX2 rabbits. Fourteen days after implantation of the experimental hepatic tumor, VX2 rabbits were treated. In group A group B and group C, the rabbits hepatic proper artery were selectively catheterized by 3 Fr microcatheters via right femoral artery under fluoroscopic guidance. 10% NSIO 0. 5 ml（ group A and group C） or Lipidol 0. 5 ml （group B） infused into proper hepatic artery. Three days after embolization, the rabbits in group A and group B were exposed to gap-type alternating magnetic field for 30 minutes, while rabbits in group C and group D have not been exposed to alternating magnetic field. The liver tumor size were measured by CT scanning before and 14 days after treatment then the animals were sacrificed, the liver, lung, heart spleen and kidney were harvested for histopathology examination, the liver tumor size were detected directly. Results All subjects experienced uneventful 14 days surivials, on the biochemical examination, there were no changes about the function of liver and renal in each group 14 days after treatment compare to pretreatment. Fourteen days after treatment, the tumor size decreased by 8.09% in group A, but increased by 9. 72% and 13.00% （P 〈0. 05） in group B and group C respectively, in group D, the tumor size increased by 57.50% （P 〈 0. 01 ）. In histopathology examination, the tumor necrosis in three treatment groups were manifest, oarticular in group A. Conclusion Arterial embolization hvperthermia with NSIO has obvious therapeutic response to experimental hepatic tumors, it encourages further development of this technology for the treatment of liver cancer in humans.