白菜核雄性不育两用系可育和不育花药ATPase的分布

ATPase DISTRIBUTION IN FERTILE AND STERILE ANTHER OF A GENIC MALE STERILE CHINESE CABBAGE

对白菜核雄性不育两用系可育花药和不育花药的ATPase做了定位分析。可育花药的花粉母细胞核中积累了大量的ATPase反应颗粒,而细胞质中ATPase反应颗粒较少,但在线粒体中特异地聚集了一些大的ATPase反应颗粒。减数分裂后,小孢子细胞质中ATPase反应颗粒明显增加。随着小孢子发育,其细胞质中ATPase反应颗粒逐渐减少,但在线粒体中又特异性地聚集了较多的AT-Pase反应颗粒。当花药发育到二胞花粉时期,花粉和绒毡层细胞中的ATPase反应颗粒已很少了。不育花药的花粉母细胞中呈现较多的ATPase,然而在线粒体中很少。异常四分体小孢子细胞质中虽然有较多的ATPase反应颗粒,但还是通过细胞质收缩和质壁分离方式退化。对可育花药的花粉母细胞线粒体中特异出现的簇状ATPase分布现象进行了分析,讨论了不育花药中花粉母细胞线粒体ATPase的异常与花粉败育的可能关系。

Lead precipitation technique was used to locate Adenosine Triphosphatase （AT- Pase）in the fertile and sterile anthers of a genie male sterile Chinese cabbage （Brassica campestris L. ssp. Chinensis Makino var. communis Tsen et Lee）,which would help us to understand the relationship between ATPase and sterility of anthers of the cabbage. At megaspore mother cell （MMC） of fertile anther many ATPase reactive precipitates were located in nucleus but few of the precipitates in cytoplasm of the cell. Meantime, some ATPase reactive precipitates also specially appeared in mitochondria of the MMC. After meiosis of MMC, the precipitates in cytoplasm of early microspores increased evidently and then decreased step by step with development. The ATPase reactive precipitates in tapetal cell also increased ultimately in early microspore stage and then decreased with development of anther. When microspore formed a large vacuole, which is late stage of microspore, the ATPase reactive precipitates were located in its mitochondria. After microspores mitosis a few of the ATPase reactive precipitates appeared in pollen grains and tapetal cells. More ATPase reactive precipitates appear in MMC of sterile anther than in fertile anther but fewer of them in mitochondria. Ahhough more ATPase granules appear in abnormal tetrad microspores which degenerate by cytoplasm shrinkage and plasmolysis. The relation between ATPase and male sterility of the cabbage was discussed.