骨形态发生蛋白-2在正常大鼠角膜成长过程中的表达

Expression of bone morphogenetic proteins-2 during development of normal rat cornea

目的确定TGF-β超家族成员骨形态发生蛋白-2(BMP-2)在正常鼠角膜生长发育过程中的表达及作用。方法用免疫组织化学方法观察TGF-β超家族成员骨形态发生蛋白2(BMP-2)分别在大鼠胚胎17d,出生当天,出生后4、7、10d和11周角膜蛋白水平的表达。每组选取3只大鼠作为实验对象。结果BMP-2在大鼠胚胎17d时就有表达,于出生当天至出生后10d及成年鼠角膜生长发育过程中呈动态变化过程。结论结果提示TGF-β超家族成员骨形态发生蛋白-2(BMP-2)在鼠角膜的表达随着角膜的生长发育而调节,BMP-2可能与角膜的生长发育及维持出生后角膜的动态平衡有关。

Objective Bone morphogenetic proteins （BMPs） are muhifunctional cytokines and members of the transforming growth factor-β（TGF-β） superfamily. Bone morphgenetic proteins have been found widely in organisms and play regulating role to cell differentiation. This study was to ascertain the role and the expression of the TGF-β superfamily bone morphgenetic proteins-2 （BMP-2） during development of the normal rat cornea. Methods Eight adult Wistar rats were used in this study. Three rats at each age were sacrificed for histological observation. Cornea were harvested from the Wistar rats,and immunohistochemistry stain was performed. Negative control was performed with PBS instead of the first antibody. The sections cut from cartilage of femoral head were stained as positive control. BMP-2 was observed at the protein level in rat cornea at aged embryonic 17 days （El7） ,at birth （P0） ,at postnatal day 4,7,10,and week 11 （P4,PT,P10 and adult） respectively by optical microscope. Results B MP-2 was expressed positively as buffy particles in corneal epithelial cells, bypotballus, subsequenee basal lamina and endodermis cells of cornea at E17 ,P0,P7 ,P10 and adult rats. There were a few buffy particles in P4 rat cornea, no buffy particle in negative control, and more buffy particles in positive control. The period from P0 to P10 and adult corresponded to the period of dynamic changes in the rat corneal development. Conclusion The expression of TGF-β superfamily BMP-2 is regulated along with corneal development and may be related to corneal development and maintain of corneal homeostasis after birth.