摘要
目的探讨人和大鼠阴茎包皮和包皮系带内降钙素基因相关肽(CGRP)免疫阳性神经末梢的分布和起源。方法采用免疫组织化学法观察成人阴茎包皮和包皮系带内CGRP免疫阳性神经末梢的分布,通过荧光金(FG)逆行标记和CGRP免疫荧光标记相结合法研究大鼠包皮系带内CGRP免疫阳性神经末梢的起源。结果成人阴茎包皮及包皮系带内均有CGRP免疫阳性神经末梢存在,主要位于表皮基底层和棘细胞层内,呈树枝状或念珠状分布,大多成束走行。阴茎系带处CGRP免疫阳性神经末梢的分布密度明显大于阴茎包皮处。大鼠的阴茎包皮和包皮系带内也有类似的CGRP免疫阳性神经末梢分布,结合FG逆行标记法研究发现,神经末梢起源于第6腰髓对应的背根神经节(L6-DRG)和第1骶髓对应的背根神经节(S1-DRG)的神经元。CGRP免疫荧光标记细胞大多为中小型,呈深绿色,沿神经束成行排列或散在分布。FG/CGRP双标阳性细胞均为中小型,其数量占FG逆标阳性细胞总数的二分之一。结论人和大鼠阴茎包皮及包皮系带内存在着大量的CGRP免疫阳性神经末梢;大鼠实验表明,这些末梢来源于L6-DRG和S1-DRG,提示CGRP可能参与阴茎包皮及包皮系带感觉信息的传递。
Objective To study the distribution and origin of calcitonin gene-related peptide (CGRP) immunoreactive nerve terminals in the prepuce and frenulum of penis of human and mice. Methods The CGRP-immunoreactive nerve terminals in the prepuce and frenulum of penis were detected by the method of immunohistochemistry. The fluoro-gold (FG) retrogradely tracing and CGRP immunofluorescence were used to tracing the origin of CGRP-immtmoreactive terminals in the penile frenulum of rats. Results In the prepuce and frenulum of adult penis, CGRP-immunoreactive nerve terminals were mainly observed in the basal stratum and spinal stratum of epidermis distributing in a cluster. These nerve terminals were arburescent and beady in appearance. The density of these nerve terminals was significantly higher in frenulum than that in prepuce. The distribution of CGRP-immunoreactive nerve terminals in rats was similar to that of in adult. FG retrogradely tracing method found that the FG retrolabelled neurons were localized in L6-DRG and S1-DRG. Combined CGRP method indicated that a number of DRG neurons were showed CGRP-immunoreactive. CGRP-positive neurons were mainly moderate or small-sized, intense immunostained, bright green granules appeared as FITC fluorescence in the cytoplasm. These positive-neurons were arranged in rows or spots among nerve bundles. A half of FG-labelled neurons also showed CGRP-immunoreactive expression in L6-DRG and St-DRG respectively. All of FG/CGRP double-labelled neurons were moderate or small-sized. Conclusion Large numbers of CGRP-immunoreactive nerve terminals were observed in the penile frenulum of human and rats. These terminals were originated from the peripheral branches of CGRP-immunoreactive neurons in L6-DRG and S1-DRG of rats. It is suggested that CGRP may take part in the transmission of nociception in the prepuce and frenulum of penis.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第4期391-395,共5页
Acta Anatomica Sinica