同型半胱氨酸对人主动脉平滑肌细胞HCY-2表达及细胞增殖的影响

EFFECTS OF HCY ON THE EXPRESSION OF HCY-2 AND THE PROLIFERATION IN HUMAN FETUS ARTERIAL SMOOTH MUSCLE CELLS

目的体外研究同型半胱氨酸(HCY)及叶酸对人主动脉平滑肌细胞(hASMCs)中同型半胱氨酸诱导基因(HCY-2)蛋白表达的影响,以及HCY对hASMCs增殖的影响。方法以免疫组织化学ABC法对体外培养的hASMCs中的HCY-2表达进行形态学观察,并利用图像分析系统对结果进行定量分析;以细胞计数法观察不同浓度HCY对体外培养的hASMCs增殖的影响。结果HCY-2主要表达在hASMCs的胞浆中,其表达强度与培养液中HCY浓度呈正性剂量依赖关系;叶酸促使HCY-2表达增强;不同浓度HCY可影响hASMCs的增殖,细胞增殖数目随HCY浓度增长而增加,当HCY到达1.25 mmol/L时,细胞数目达到最大值,随着HCY浓度的继续增加,细胞增殖呈下降趋势。结论HCY促使HCY-2表达增强;并影响hASMCs的增殖。叶酸对HCY有拮抗作用。

Objective To study the homocysteine induced gene （HCY-2） expression in human fetus arterial smooth muscle cells （hASMCs） in culture and explore the effects of homocysteine （HCY） and folic acid on HCY-2 expression and cell proliferation of hASMCs. Methods Immunohistochemistry ABC staining method was used to observe and analyze HCY-2 expression in hASMCs in culture. The image analysis system was used to research of hASMCs quantificationally. The effect of different HCY concentration on the proliferation of hASMCs was investigated by the cell counting. Results Immunoreactive substance of HCY-2 was chiefly found in cytoplasm of hASMCs. The expression of HCY-2 could be affected by HCY concentrations. There was a positive dose-dependent correlation with HCY concentrations in the culture medium. Folic acid increased the expression of HCY-2. The different concentration of HCY enhanced the proliferation of hASMCs, and this enhancement was maximal at the concentration of 1. 25 mmol/L of HCY, while the proliferation was decreased when the concentration of HCY was over 1.25 mmol/L. Conclusion HCY increases the expression of HCY-2, and affects the proliferation of hASMCs. HCY is inhibited by folic acid.