不同鼠龄大鼠心肌组织中钙敏感受体的表达及与缺氧-再灌注损伤的关系

Expression of calcium-sensing receptor in the rat cardiac tissue at different age and its relation with anoxia-reperfusion injury

目的:观察不同鼠龄大鼠心肌组织钙敏感受体(CaSR)的表达规律及与心肌缺氧-再灌注损伤的关系。方法:采用RT-PCR技术,检测不同情况下心肌组织CaSR的表达差异;Lagendorff离体心脏灌流的方法复制心脏缺氧-再灌注模型;用透射电镜观察心肌超微结构的变化。结果:大鼠出生后,心肌组织中CaSR的表达逐渐升高,1个月时达到高峰,2、3个月保持在出生水平,此后上升并维持在较高水平。大鼠心肌缺氧40 m in及再灌注1 h、2 h心肌组织CaSR的mRNA表达升高,再灌注3 h、4 h后降低;同时随再灌注时间延长,心肌超微结构损伤愈严重。结论:大鼠心肌组织存在钙敏感受体,其表达与月龄有一定关系,并可能参与心肌缺氧-再灌注损伤的发生。

AIM： To investigate the changes of expression of calcium - sensing receptor （CaSR） in the rat cardiac tissue at different age and its relation with anoxia - reperfusion （A/R） injury. METHODS ： RT - PCR was used to detect the mRNA expression of CaSR. The A/R injury was remodeled in vitro, and the ultrastructure of cardiac tissue was observed under transmission electron microscope. RESULTS： The expression of CaSR mRNA in the rat cardiac tissue increased gradually after birth, and reached to its maximum in the 1 st month. In the 2nd to 3rd month, the expression were almost equal to that at birth and then increased again to a higher level. The expression level of CaSR mRNA increased more significantly than those in control groups after 40 min of anoxia and repeffusion of 1 h and 2 h, and decreased after 3 h and 4 h. Moreover, the longer the reperfusion time lasted, the more serious the changes of ultrastructure were observed. CONCLUSION： Our results demonstrate that the CaSR is expressed in the rat cardiac tissue. The mRNA expression of CaSR changes with age and is involved in the anoxia -repeffusion injury.