葛根素对肺缺血-再灌注损伤时Fas/FasL表达的影响

Effect of puerarin on expression of Fas/FasL mRNA in lung tissue with pulmonary injury induced by ischemia-reperfusion in rabbits

目的:探讨葛根素对肺缺血-再灌注损伤(PIR I)时Fas/FasL表达的影响。方法:采用在体兔单肺原位缺血-再灌注模型。实验兔70只,随机分为假手术对照组(sham,10只)、肺缺血-再灌注组(I-R,30只)和肺缺血-再灌注加葛根素组(LGT,30只)。每组又分为再灌注1 h、3 h、5 h 3个亚组,每个亚组10只,分别于再灌注1 h、3 h、5 h 3个时点取左肺组织,观察Fas/Fas配体(Fas/FasL)mRNA定位表达、凋亡指数(AI)、肺组织湿干重比(W/D)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变。结果:肺再灌注1 h、3 h、5 h,LGT组Fas/FasL mRNA在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达,明显低于同一时点I-R组(P<0.05);AI、W/D和IQA值显著低于I-R组(P<0.01和P<0.05);肺组织形态学异常改变不同程度减轻。结论:葛根素可下调肺组织Fas/FasL mRNA的表达而减轻细胞凋亡,对PIR I发挥积极的防治作用。

AIM： To investigate the effect of puerarin （Pur） on expression of Fas/FasL mRNA m lung ussue during pulmonary ischemia and reperfusion injury （PIRI） in rabbits. METHODS： Single lung isehemia and reperfusion animal model was used. The rabbits were randomly divided into three groups, sham operated group （sham, n = 10）, PIR group （I - R, n =30） and PIR ＋ Pur group （Pur, n =30）. Changes of several parameters included apeptotic index （AI）, wet to dry ratio of lung tissue weight （W/D） and index of quantitative assessment of histologic lung injury （IQA） were measured at 60, 180 and 300 minutes after reperfusion in lung tissue. Meanwhile, the location and expression of Fas/FasL mRNA were observed. Lung tissue was prepared for light microscopic and electron microscopic observation at 60, 180,300 minutes after reperfusion. RESULTS： As compared with group I - R, Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia in group Put. The values of AI, W/D and IQA showed significantly lower than that in group I - R at 60, 180, 300 minutes after reperfusion in lung tissue （ P 〈 0. 01 and P 〈 0. 05 ）. Meanwhile, abnormal changes of the lung tissue in morphologically were lessen markedly in group Put. CONCLUSION： Puerarin produces a notable protective effects on PIRI in rabbits by inhibiting Fas/FasL mRNA expression and decreasing apeptosis.