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SARS-CoV抗原免疫鸡后卵黄抗体效价变化研究 被引量：1

Change of yolk antibody titers in hens immunized with SARS-CoV antigen
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摘要 目的:了解SARS-CoV抗原免疫鸡后,卵黄抗体效价的变化。方法:卵黄1∶1 600倍稀释后ELISA方法测定抗体效价。结果:Cutoff为0.21±0.05,在首次免疫26-28 d后,抗体效价达到最高(1∶1 600稀释(A450=1.3),并且抗体持续高效价(A450>0.8)。随着免疫时间的延长,抗体效价降低;间隔一定时间加强免疫,抗体效价又回升达到高峰。结论:SARS-CoV抗原持续免疫鸡,卵黄抗体持续保持高效价。 AIM ： To observe the change of yolk antibody titers after immunizing hens with SARS - CoV antigen. METHODS： Yolk antibody diluted with water to 1 ： 1 600 was determined with ELISA methods. RESULTS ： The Cutoff is 0. 21 ±0. 05. After immunization, antibody titers in hens reached the peak in first 26 -28 day （A450 = 1.3 when antibody was diluted as 1：1 600）, and hold high titers （A450 〉0. 8）. The antibody titers decreased after the time of immunity. The increase and peaking of antibody titers were observed at intervals when repeated immunizations were given. CONCLUSION ： Yolk antibody kept high titers when hens were immunized with SARS -CoV antigen.
作者 何小维 彭运平 王继华 李文美 罗志刚
机构地区 华南理工大学轻工与食品学院 华南理工大学万孚生物技术有限公司
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2006年第8期1633-1635,共3页 Chinese Journal of Pathophysiology
基金 广东省防治非典型肺炎科技攻关项目(粤科企[2003]156号) 国家自然科学基金资助项目(No.29774009) 广东省自然科学基金资助项目(No.970468)
关键词 冠状病毒属 卵黄襄 抗体 Coronavirus Yolk sac Antibodies
分类号 R363 [医药卫生—病理学]
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参考文献9

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共引文献3

同被引文献16

  • 1余清声,王桂平,王薇,覃媛,黄劭.抗眼镜王蛇毒鸡卵黄抗体的研制及初步应用[J].中华微生物学和免疫学杂志,2004,24(9):695-698. 被引量：12
  • 2刘四红,孔天翰.鸡血清与卵黄中抗中华眼镜蛇毒IgY动态变化研究[J].蛇志,2006,18(4):261-264. 被引量：2
  • 3刘四红,孔天翰.抗眼镜蛇毒鸡卵黄抗体的制备、纯化和保护性实验研究[J].中国病理生理杂志,2006,22(12):2453-2457. 被引量：7
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中国病理生理杂志
2006年 第8期

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