两种精浆酸性磷酸酶检测方法的比较与评价

Comparison and Evaluation of Two Methods for Measureing Acid Phosphatase Activity in Seminal Plasma

目的:对检测精浆酸性磷酸酶(ACP)活性的常规方法(磷酸苯二钠法)和试剂盒方法进行比较,并探讨试剂盒方法取代常规方法用于常规检测的可能性。方法:79份不育男性的精浆,分别用常规方法和试剂盒方法检测其ACP活性。取1份精浆标本用于两种方法的批内检测,另取2份标本用于两种方法的批间检测。随机留取10份精浆标本,稀释后立即或放置30 m in后检测ACP活性。另随机留取10份精浆标本,同时由2位技术人员分别用常规方法检测ACP活性。结果:试剂盒方法所测ACP活性与常规方法显著相关(r=0.745,P=0.000)。试剂盒方法与常规方法相比,两者批内CV(13.72%;10.66%)比较接近,而批间CV(13.8%和15.49%;24.43%和21.04%)明显较低。精浆稀释后放置30 m in,无论是常规方法还是试剂盒方法,所测结果都显著降低(P<0.05)。而2位技术人员用常规方法所测精浆ACP活性无显著区别(P=0.165)。结论:试剂盒方法检测精浆ACP活性优于常规方法,可以取代常规方法。

Objective： To compare the routine method and the kit method for the measurement of acid phosphatase activity in seminal plasma, and to explore the possibility of the kit method for routine measurement. Methods： Seventy-nine seminal plasma samples were assayed by routine method and kit method respectively for acid phosphatase. One sample was detected 10 times for within-run analysis, and an other two were measured by both the methods once a day for 10 days for between-run analysis. Acid phosphatase activities in another 10 seminal plasma samples collected at random were measured immediately or 30 min after dilution by two technicians, respectively. Results： There were significant positive correlations between the acid phosphatase activities measured by routine and kit methods （ r = 0. 745, P = 0. 000）. In the within-run assay, the coefficient of variation for the kit method （ 13.72% ） was similar with that for the routine method （ 10.66% ）. But in the between-run assay, the coefficients of variation for the kit method （ 13.8% and 15.49% ） were obviously lower than those for the routine method （24. 43% and 21. 04% ）. Compared with the acid phosphatase activities in seminal plasma measured immediately after dilution, those measured after 30-min standing were notably lower for either of the methods （ P 〈 0.05 ）. However, there wasnt significant difference in the acid phosphatase activities detected by the routine method between the two technicians （ P = 0. 165 ）. Conclusion ： The kit method is superior and preferable to the routine method for the measurement of acid phosphatase in seminal plasma.