HLA-DRB1,DQA1等位基因与广西壮族系统性红斑狼疮器官系统损害相关性研究

Relationship between HLA-DRB1,DQA1 Locus and Organ,System Involovement in Patients with Systemic Lupus Erythematosus of the Zhuang Nationality in Guangxi

目的分析HLA-DRB1,DQA1等位基因与广西壮族系统性红斑狼疮(SLE)器官系统损害的相关性。探讨广西壮族SLE从基因到临床表达的发病机制。方法应用聚合酶链式反应-序列特异性引物(PCR-SSP)技术检测128例确诊的SLE患者的HLA-DRB1,DQA1等位基因,并与患者的各种临床指标进行统计学分析。结果蛋白尿与DRB1*15和DRB1*16负相关,与DQA1*0301正相关;血尿素氮增高与DRB1*1401和DQA1*0501负相关;C3降低与DRB1*1401负相关,与DQA1*0101正相关;白细胞减少与DRB1*16负相关;贫血和淋巴细胞减少与DQA1*0102正相关;血小板减少与DQA1*0301负相关;面部红斑与DQA1*0101负相关;关节痛与DQA1*0104正相关。结论多个HLA-DRB1,DQA1等位基因与广西壮族SLE临床表现有相关性。广西壮族SLE遗传背景与其他人种有所不同。

Objective To explore the relationship between HLA-DRB1, DQA1 and involvement in patients with systemic lupus erythematosus （SLE） of the Zhuang nationality in Guangxi. Methods Polymerase chain reaction-sequence specific primers technique （PCR-SSP） was used to type HLA-DRB1, DQA1 in 128 patients with SLE of Zhuang nationality in Guangxi. Using statistical analysis to analyse the relationship between HLA-DRB1, DQA1 and clinical indexes. Results Proteinuria was negatively associated with DRB1 ＊ 15,DRB1 ＊ 16 respectively, positively with DQA1 ＊ 0301 ;increased BUN negatively with DRB1 ＊ 1401, DQA1 ＊ 0501 respectively; decreased C3 negatively with DRB1 ＊ 1401, positively with DQA1 ＊ 0101 ;leukocytopenia negatively with DRB1 ＊ 16;anemia and lymphocytopenia respectively positively with DQA1 ＊ 0102;thromboeytopenia negatively with DQA1 ＊ 0301 ;butterfly erythema negatively with DQA1 ＊ 0101 ; arthralgia positively with DQA1 ＊ 0104. Conclusion Many HLA-DRB1, DQA1 locus play an important role in the clinical expression of SLE of the Zhuang nationality in Guangxi, and the genetic background of SLE of Zhuang nationality in Guangxi may be different from other race.