脓毒症患儿中性粒细胞、淋巴细胞CD11b表达的研究

Study on CD11b expression of neutrophils and lymphocytes in pediatric sepsis

目的探讨脓毒症患儿中性粒细胞、淋巴细胞CD11b的表达意义。方法用流式细胞术检测脓毒症(观察组)患儿27例中性粒细胞、淋巴细胞CD11b表达,并与对照组20例和正常组20例比较。结果在急性期、恢复期,观察组中性粒细胞CD11b[(98·26±1·55)%,(97·74±1·41)%],与对照组[(86·3±6·33)%,(81·48±3·35)%]和正常组[(69·59±9·98)%]比较,P<0·05。急性期,观察组淋巴细胞CD11b[(15·17±10·2)%],低于对照组[(19·2±7·6)%],P<0·05;严重脓毒症淋巴细胞CD11b[(6·54±2·52)%]表达下调,低于脓毒症组[(19·45±8·68)%],P<0·05。结论中性粒细胞、淋巴细胞CD11b表达在脓毒症的演变过程中起到一定的作用,可作为脓毒症的判断依据,预测疾病的发展。

Objective To explore significance of CDllb expression on neutrophils and lymphocytes in pediatric sepsis. Methods Based on flow cytometry, CDllb expression on neutrophils and lymphocytes in 27 cases with pediatric sepsis, 21 cases of control group and 20 cases of normal group were measured and then compared. Results in acute and recovery period, neutrophil CDllb expression in sepsis group were [ （98.26 ±1.55） %, （97.74 ± 1.41） % ], higher than those of control group[ （86.3 ±6.33） %, （81.48 ± 3.35） % ] and normal group [ （69.59 ± 9.98） % ], P 〈 0.05 respectively. In acute period, lymphocyte CD11b expression of sepsis group was lower than that of control group [（15.17 ± 10.2）% ver （19.2 ± 7.6）%], P〈0.05. However, lymphocyte CD11b expression in severe sepsis was lower than that of sepsis [ （6.54 ± 2.52） % ver （19.45±8.68）%],P〈0.05.Conclusion CD11b expression on neutrophils and lymphocytes can affect pediatric sepsis prognosis. It can be a candidate marker of sepsis and a useful parameter for prognosis of the disease.