2004年11月-2005年3月北京地区儿童流行性感冒病原学检测分析

Etiological analysis of pediatric influenza in Beijing from November,2004 to March,2005

目的对北京地区2004年11月～2005年3月流行性感冒患儿进行病原学检测及分析。方法狗肾传代细胞（MDCK）进行流感病毒分离，用血凝试验、血凝抑制试验进行型和亚型的鉴定；用快速酶联免疫法检测A型及B型流感病毒抗原试剂盒检测患儿咽拭子中的流感病毒抗原。结果217例患儿中111例检测出流感病毒，检出率为51％（111／217），流感病毒A型28例（28／111），占检出数的25．3％，流感病毒B型82例（82／tit），占检出数的73．8％，流感病毒A＋B型1例（I／111）。占检出数的0．9％。111例流感病毒检测阳性患儿中病毒分离与快速诊断二项同时阳性75例。二者阳性符合率67．5％。结论北京地区2004年11月～2005年3月流感流行季节流感病毒以B型为优势株。流感快速诊断试剂盒可快速、准确地检测出流感病毒及型别。

Objective To analyze the pathogen of pediatric influenza in Beijing from November, 2004 to March, 2005. Methods The influenza virus isolation was performed by MDCK cell culture. The isolate identification was carried out with hemagglutination test（HA） and hemagglutination inhibition tests （HI）. The throat swab specimens were detected using the rapid Flu A ＋ B antigens kit with enzyme immunoassay （EIA）, Results Influenza viruses were isolated and/or detected from 111 cases of 217 children （total positive rate was 51% ）. There were 28 strains of influenza A virus （28/111,25.3 % ）, 82 strains of influenza B virus （82/ 111,73.8 % ） 1 strain of influenza A and B virus （ 1 / 111,0.9 % ）. The influenza virus isolation and rapid EIA test were both positive in 75 cases of the 111 patients （67.5 % ）. Conclusion The dominant strain of influenza virus was influenza B-virus in Beijing from November, 2004 to March, 2005. The rapid Flu A ＋ B antigen kit with enzyme immunoassay is a rapid and effective method of influenza virus detection and subtype identification.