三叶木通籽亚麻酸的提取分离研究

Study on flax and acid draw separate of Akebia trifoliata seed

以提取与纯化三叶木通亚麻酸为目的,采用不同溶剂提取脂肪酸,探讨脱蛋白的乙醇浓度以及脱色时使用活性炭的比例,筛选脂肪酸脱蛋白和脱色的最佳条件,达到初步除杂的目的。同时进行了冷冻结晶的正交实验,进一步纯化亚麻酸,并采用气相色谱法检测。结果表明:石油醚是三叶木通籽提取亚麻酸的最好溶剂,粗脂肪的得率为31.8%,γ—亚麻酸的含量为35.42%,粗脂肪酸经过脱色、脱蛋白初步除杂后,γ—亚麻酸含量有所提高,经过冷冻结晶纯化后,γ—亚麻酸含量可达42.40%。

In order to draw purification Akebia trifoliata coherent sour as the purpose flax, adopt and does not draw the fatty acid with the solveto, Through is it take off ethanol density of albumen and use active proportion of charcoal when decolouring to probe into. Define the optimum condition that the fatty acid takes off albumens and decolours. Achieve except that miscellaneous purpose preliminarily. Carry on cold to freeze paying experimenting brilliant, further purification flax sour, adopt the gaseous phase to measure. The result shows： Petroleum ether whether Akebia trifoliata seed put through draw the sour best solvent of flax. To get rate thick fat is 31,8 %, γ-the content of the flax and acid is 35.42% ,Through decolour,take off albumen preliminary divided by after mixing thick fatty acid at the same time, γ-the sour content of the flax improves to some extent, After it is cold to freeze the brilliant purification, γ-flax sour content high to a little, can up to 42.40 %.