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成人隐匿性自身免疫性糖尿病患者CD4^+CD25^+T细胞的变化研究 被引量：7

Changes of CD4^+CD25^+T cells in patients with Latent autoimmune diabetes in adults
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摘要 目的:通过观察成人隐匿性自身免疫性糖尿病(LADA)患者CD4+CD25+T细胞的变化并与速发性1型糖尿病(T1DM)比较,了解成人隐匿性自身免疫性糖尿病患者T细胞免疫功能的变化及与1型糖尿病的异同点。方法:LADA组24例,速发性T1DM18例,对照组20例,应用流式细胞技术测定3组入选者T细胞表面分子CD4、CD8、CD25、CD4+CD25+、CD8+CD25+、CD4+CD25+CD62L+,以百分比表示各表面分子阳性T细胞占外周血淋巴细胞的比例。结果:LADA与T1DM组CD4+T细胞、CD4/CD8比值明显高于健康对照组(P<0.01),LADA与T1DM对比无差异。LADA组CD25+、CD4+CD25+、CD4+CD25+CD62L+T细胞高于健康对照组(P<0.05),明显高于T1DM组(P<0.01)。T1DM组CD25+、CD4+CD25+、CD4+CD25+CD62L+T细胞略低于健康对照组,但无统计学意义(P>0.05)。结论:LADA患者外周血中诱导免疫耐受的CD4+CD25+、CD4+CD25+CD62L+T细胞较对照组升高并明显高于T1DM患者。LADA患者胰岛β细胞功能下降较速发性T1DM患者相对缓慢可能与CD4+CD25+T细胞的免疫保护有关。 Objective：To observe the changes of CD4^＋ CD25^＋T ceils in patients with Latent autoimmune diabetes in adults. Methods ：The lymphocyte subpopulations（ CD4^＋ ,CD8^＋ , CD25^＋ ,CD4^＋ CD25^＋ ,CD8^＋ CD25^＋, CD4^＋ CD25^＋ CD62L^＋ T cells ） in peripheral blood mononuelear were in 24 LADA, 18 type 1 diabetic with rapid progression, and 20 normal control by flow cytometric. We assessed the immune changes of T cells subsets of LADA and compared with type 1 diabetic with rapid progression. Results ： （ 1 ） Patients with LADA and typel diabetes had higher CD4^＋ T cells and CD4^- CD8^- ratio compared with patients type 2 diabetes （ P 〈 0. 05）. There were no differences between LADA and type 1 diabetes（ P 〉 0.05 ）. （2）The CD25^＋, CD4^＋ CD25^＋, CD4^＋ CD25^＋ CD62L^＋ T cells in LADA patients was higher than normal control（ P 〈 0.05 ）, and more higher than type 1 diabetes （ P 〈 0.01 ）. Conclusion ：The increasement of regulatory T cells may play an important protective role in development of LADA.
作者 石昌红 管庆波 高聆 马春燕 崔彬 刘国立 赵家军
机构地区 山东大学山东省立医院内分泌科
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2006年第8期771-774,共4页 Chinese Journal of Immunology
基金 山东省医药卫生科技发展计划项目(199940)
关键词 UADA(成人隐匿性自身免疫性糖尿病) CD4^+CD25^+T细胞 流式细胞术 Latent autoimmune diabetes in adults CD4^＋ CD25^＋ T regulatory cells Flow cytometric
分类号 R587.1 [医药卫生—内分泌]
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参考文献8

  • 1Camara N O,Ng W F,Hernandes-Fuentes M et al.Human CD4^+ CD25^ + T cells regulate CD8^ + T cells activation[J].Transplant Proc,2002;34(20):2858-2860.
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  • 3施毕旻,成兴波,於葛华,张学光.1型糖尿病外周血淋巴细胞CD_(28)、4-1BB共刺激分子的表达与胰岛功能的相关性[J].中华内分泌代谢杂志,2003,19(1):48-49. 被引量：4
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  • 6郑峻松,吴军,易绍萱,贺伟峰,陈希炜,解志杰,彭双发,代飞.CD4^+CD25^+T_(reg)细胞调节T细胞的作用机制分析[J].第三军医大学学报,2003,25(21):1881-1884. 被引量：18
  • 7Green E A,Choi Y,Flavell R A.Pancreatic lymph node-derived CD4^+ CD25^ + Treg cells:highly potent regulators of diabetes that require TRANCE-RANK signals[J].Immunity,2002; 16(2):183-191.
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二级参考文献13

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  • 10郑峻松,吴军,马兵,罗高兴,易绍萱,贺伟峰,陈希炜.CTLA4Ig基因转染猪皮异种移植的体外实验研究[J].第三军医大学学报,2001,23(7):762-764. 被引量：13

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中国免疫学杂志
2006年 第8期

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