毛白杨与珠美海棠试管苗低温贮存技术初探

A preliminary study on the technology for storage of tissue-cultured plantlets in low temperature condition

林木试管苗通过0～10℃的自然低温贮存后,具有较高的成活率且抗冷性明显提高。观察和分析不同贮存阶段的试管苗形态和生理指标的变化,结果表明,试管苗随着贮存温度的降低,淀粉呈下降趋势,而可溶性糖相应上升,粗蛋白含量波动起伏略有升高,这是试管苗适应低温和弱光照的生理反应。贮存165d的分化苗和120d的生根苗污染损失率均低于10.0％;分化苗的有效苗数显著增加,生根率在95.5％以上,生根苗的移栽成活率为93.5％～98.5％。

After stored in natural low temperature (0~ 10 C) , forest tree tissue-cultured plantlets' had a higher survival rate, their chilling resistant ability was increased visibly.The results from observing and analysing the morphological and physiological characters of the platlets at different storage period showed that with the decreasing of storage temperature,starch content was decreased, while the soluble sugar content increased,crude protein content was increased slightly with fluctuation,which are the physiological reactions that the tissue-cultured plantlets adapted to low temperature and weak light.The losing rates after storage for the 165 days dif-ferented plantlets and 120 days rooted plantlets were all lower than 10.0%.After the storage the effective number of the differented plantlets was greatly increased with more than 95.5% rooting rate and the transplanting survival rate of the rooted plantlet reached 93.5%-98.5%.