人芽囊原虫在199单相培养基中的体外培养

Culture of Blastocystis hominis in vitro in Medium 199

目的 观察199单相培养基对人芽囊原虫（Blastocystis hominis）的体外培养情况。方法 采用199单相培养基对B．hominis进行体外连续培养，比较不同pH值、血清种类及接种量等条件下虫体生长繁殖情况及影响因素。结果B．hominis在199培养基中最适培养条件为：10％～20％小牛、人或马血清；接种量不小于1×10^5原虫／管；青、链霉素于37℃条件下厌氧培养；pH为7．0、7．5及8．0时，B．hominis繁殖高峰分别为第4、8天，第3、6、9天和第2、4、7、9天。结论 使用199单相培养基可以对B．hominis进行体外长期培养。

Objective To establish the culture of Blastocystis hominis in vitro in Medium 199 for further research on the biological characteristics of this intestinal parasite. Methods The growth, reproduction and relevant factors of B. hominis under different culture conditions including pHs, sorts and concentrations of serum, and the number of inoculation were compared. Results The op- timal conditions for the continuously anaerobic culture of B. hominis in Medium 199 were as follows： concentrations of new born bovine serum （or human serum and horse serum） ranged from 10%- 20%, the number of inoculation was no less than 1 × 10^5 organisms per tube, pHs ranged from 7.0 -8.0, penicillin, streptomycin should be added, anaerobic cultured at 37 C. The reproductive peaking-days were 4, 8atpH 7.0; 3, 6, 9atpH 7. Sand 2, 4, 7, 9atpH 8.0. Conclusion B. hominis could be continuously cultured in vitro in Medium 199.