摘要
以番茄为试材,经2.4kJ/m2短波紫外线(UV-C)照射,取果皮经冰浴研磨、硫酸铵分级沉淀、凝胶过滤和离子交换层析,分离纯化苯丙氨酸解氨酶。经测定,紫外照射组PAL活性为81.0U/mL,比对照组高5U/mL,分离纯化倍数为16.4倍,纯化获得的酶经SDS—聚丙烯酰胺凝胶电泳分析,其亚基分子量约为76kD。
Phenylalanine ammonia-lyase （PAL） of tomato peel were induced after 2.4 kJ/m^2 ultraviolet light-C （UV-C） irradiation. PAL was separated and purified By ice rubbing, sulfate grade deposition, gel filtration and ion exchange chromatography. Induced PAL activity was 81.0 U/mL, more than 5 U/mL to control group. Purification multiple was 16.4. Rarefied enzyme by SDS-PAGE became singleness albumen strip. Its subunit molecular weighted about 76 kD.
出处
《农产品加工（下）》
2006年第8期67-70,74,共5页
Farm Products Processing
