嗜热拟青霉固体发酵产木聚糖酶的纯化和应用研究

The Research of Purification and Application of Xylanase Produced by Paecilomycesthermophila under Solid-state Fermentation

利用一株拟青霉新种嗜热拟青霉J18,以天然农业废弃物小麦秸杆为碳源固体发酵产木聚糖酶,优化发酵条件后,产木聚糖酶水平达到16500U/g干基碳源。粗酶液经硫酸铵沉淀、凝胶过滤层析和离子交换层析3个步骤纯化,得到了电泳级纯酶。研究了该酶对馒头品质的影响,发现在面团中添加木聚糖酶能够明显改善面团的操作性能,提高馒头的比容,改善馒头的组织结构,延缓馒头的老化。

A new thermophilic Paecilomyces strain i.e. Paecilomyces thermophila J18 was used to produce xylanase under solid-state fermentation using wheat straw as the carbon source. After optimizing of the fermented conditions, the xylanase activity was up to 16 500 U/g dry substrate. The xylanase was purified through ammonium sulfate precipitate, gel filtration and ion-exchange chromatography. Then, the effect of the xylanase on the Chinese steamed bread was investigated. The results show that the addition of xylanase to the dough could obviously improve the quality of the streamed bread.