摘要
以甘蓝型油莱奥罗（Ｏｒｏ）为受体，诸葛菜（Ｏｒｙｃｈｏｐｈｒａｇｍｕｓｖｉｏｌａｃｅｕｓ）为供体，用花粉管通道法，在自花授粉２４小时后导入诸葛菜总ＤＮＡ，其后代发生了显著变异。变异率达９．８％。分析比较变异株ＤＩ－１０和Ｏｒｏ的经济性状表明，主花序有效长度增加２９．８％，第一次有效分枝数增加３３．３％，有效荚果数增加１７６．９％，每荚粒数增加１８．５％，千粒重增加４４．２％，单株产量增加３．６７倍；含油量增加２．４２％，油酸含量增加１４．３３％，芥酸减至０．６８％。Ｓｏｕｔｈｒｅｎ杂交表明，转化油菜（ＤＩ－１０）产生优良遗传特性是诸葛菜ＤＮＡ导入所致。
Exogenous total DNA extracted and purified from the donor (Orychophragmus violaceus) was microinjected into the ovary of B. napus (ORo)through the pollen tube channel 24hr after artificial pollination, and 9. 8% offsprings varied. The analyses of economic characters for varied plant (D10) showed that valid length of main inforesence increased by 29. 8%;No. of lst valid branches increased by 33. 3 % ; no. of valid pod increased by 176. 9% ; seeds. per pod increased by 18. 5 % ;1000-grain weight increased by 44. 2% (g) ; yield /plant increased by 3. 67 times. and the oil contents increased by 2.42%. Meawhile, the contents of oleic acid increased by 14. 33% ; erucic acid decreased to 0. 68%. Southren blot showed that orychophrgmus violaceus DNA's intervention were induced to varied B. napus, which proved that O. vidaceus DNA was superior character recipient.
出处
《西南农业学报》
CSCD
1996年第4期35-41,共7页
Southwest China Journal of Agricultural Sciences
基金
四川省科委资助项目
关键词
诸葛菜DNA
奥罗油茶
花粉管通道法
杂交
Orychophragmus Violaceus DNA
B. nap us
Pollen tube channel
Superior character
Southren blot
