豆科树种根瘤菌与四种豆科植物的接种试验

Experiment on the Inoculation Test between Rhizobia from Woody Legumes and Four Leguminous species

从５１种豆科树种（含羞草亚科５属，蝶形花亚科１８属）根瘤中分离获得的６６个菌株与４种豆科植物（刺槐、合欢、大豆、豌豆）进行接种试验，幼苗经５０ｄ培养，刺槐、合欢、大豆、豌豆的结瘤率分别为１００％、８７．９％，６９．７％、２５．８％。其中有１２个菌株可同时在这４种豆科植物上结瘤，２９个菌株可同时在刺槐、合欢和大豆上结瘤，４个可同时在刺槐、合欢和豌豆上结瘤，说明豆科树种根瘤菌具有较广泛的共生寄主范围。各菌株与不同豆科植物的亲合力、结瘤特性和固氮能力有较明显差异。观察还进一步证实根瘤的形状和颜色是由寄主植物决定的。

rhizobial strains,isolated from root nodules of 51 species of woody legumes(5 genera in subfamily Mimosoideae and 18 genera in subfamily Papilionoideae) were inoculated with two tree legumes ( Robinia pseudoacacia,Albizia julibrissin )and two herbaceous legumes ( Glycine max, Pisum sativum ). 50 days after cultivation of legume seedlings,the nodulation rates of the locust,silk tree,soybean and pea were 100%,87.9%,69.7% and 25.8% respectively.Among all the rhizobial strains 12 were nodulated on all of the 4 leguminous species,29 on locust, silk tree and soybean,and 4 on locust,silk tree and pea simultaneously.It has been shown that the root nodule bacteria of woody legumes have a wide range of hosts,but each rhizobial strain makes a great difference in affinity with different legumes,nodulation characters and nitrogen fixing capacity.It seems that the host plant usually determines the shape and color of the nodules.