流式细胞仪检测粒细胞抗体在白细胞减少症中的应用

Flowcytometry for Detection of Granulocyte Associated Antibody(GAA) in Patients with Leukocytopenia

目的:为探讨白细胞减少症患者粒细胞相关抗体水平,分析其临床应用价值。方法:采用流式细胞术(FCM)测定了345例白细胞减少症患者及60名正常人粒细胞相关免疫球蛋白(Ig)。结果:发现,初治345例白细胞减少症患者中粒细胞相关抗体(IgG、IgM、IgA)在粒细胞表面阳性百分率均高于正常对照组(P<0.01),约82.02%的白细胞减少症患者粒细胞抗体阳性。结论:FCM用于白细胞减少症患者的粒细胞相关抗体的检测,具有快速灵敏、简便、用血量少和结果准确的特点,对临床治疗白细胞减少症提供依据。

Objective To investigate the applicability of flowcytometry for detection of granulocyte associated antibody （GAA） in patients with leukopenic. Methods The positive rate of granulocyte associated antibody （Igs） was determined with flowcytometry in 345 patients with leukocytoponia and 60 controls. Results Positive rates of all three GAA Igs （ IgG, IgM, IgA） were significantly higher in the patients than those in the controls （ P 〈0.01 ）. GAA was positive in 82.02% of the leukopenic patients. Conclusion Detection of GAA with flowcytometry is simple, sensitive and accurate. Only a small amount of blood is required for this test.