明胶海绵复合重组人成骨蛋白-1的骨修复材料免疫原性及肌肉刺激性实验研究

Study on immunogenicity and muscular stimulation of rhOP-1 bone repairing material embedded in gelatin sponge

目的考察以明胶海绵为载体的重组人成骨蛋白-1(rhOP-1)骨修复材料的免疫原性及肌肉刺激性。方法以明胶海绵为载体制备rhOP-1骨修复材料,间接ELISA方法观察该骨修复材料植入小鼠体内的免疫原性反应,病理切片观察其对周围肌肉的影响及形态变化。结果ELISA结果表明,此法制备的以明胶海绵为载体的rhOP-1骨修复材料未引起小鼠的特异性抗体产生。植入部位肌肉虽出现炎细胞浸润现象,但炎症反应呈可逆性,未发现肌肉组织的坏死,且组织钙化现象明显。结论明胶海绵复合rhOP-1的骨修复材料在免疫原性和肌肉刺激性方面安全可靠。

Purpose To study the immunogenicity and muscular stimulation of rhOP-1 bone repairing material embedded in gelatin sponge in vivo. Methods The indirect ELISA method was used to study the immunogenicity, and the pathological section method was used to study the musclular affection of implant and morphologic changes. Results The immunogenicity test results showed that the rhOP-1 bone repairing material can not induce the production of the corresponding antibody. The inflammatory cell infiltrated, but the inflammatory reaction was reversible and no necrosis was observed in implanted muscle. Conclusion The animal experiment results showed that the rhOP-1 bone repairing material embedded in gelatin sponge was safe in the immunoenicity and muscular stimulation.