槲寄生内生菌发酵产物抗平滑肌细胞增殖和抗菌作用初步研究

The Studies of Pharmacological Action of Ferment Product of Endophytic Fungi Isolated from Viscum Coloratum on VSMC Proliferation and Antimicrobial Activity

目的:初步研究槲寄生内生真菌发酵产物的药理作用,为解决槲寄生等寄生药用植物的资源短缺问题提供新的研究思路。方法:从槲寄生中分离出内生真菌,对发酵产物进行了平滑肌细胞增殖的影响和对细菌的抑制作用实验研究。结果:从槲寄生中分离到JF、A1、G6等11株内生真菌;G6对牛主动脉平滑肌细胞(VSMC)增殖有抑制作用;JF、A1发酵产物具有较广谱的抗菌活性,对革兰氏阳性细菌如表皮葡萄球菌,革兰氏阴性细菌如产气肠杆菌、大肠杆菌、伤寒杆菌等均具有较强的抑制作用。结论:槲寄生内生真菌发酵产物同槲寄生一样具有抗动脉粥样硬化作用,并且还具有明显的抑菌作用,值得进一步深入开发研究。

Objective： Studies the pharmacological action of ferment product of endophytic fungi isolated from Fi＇scum coloratuna, provides a novel way for solving the resource shortage of Viscum coloratum and the llke autoeciousness drug plant. Methods： Several endophytic fungi were isolated from Viscum coloratum. And the primary pharmacological action of ferment products were studied by using the method of MTT assay and filter paper assay. Results： Eleven endophytic fungi were isolated from Viscum coloratum, three strains（JF, Aland G6） were investigated in detail. The ferment product of strains JF and Al with a broad spectrum ofantimicrobial activity, against one or more sensitive microbes, such as Staphylococcus epldermidis （ G ＋ ）, Enterobacter aerogenes, Escherichia coli and Typhoid bacillus（ G- ） etc, were screened out using the filter paper assay, strain C,6 have inhibiting effects on VSMC proliferation. Conclusion：Viscum Coloratum endophytic fungi fermentation broth has important pharmacological action such antiatheresclerotic and antimicrobial activity ctc,just as Viscum coloratum possess. Further study on its activity will be carried out in the future.