硫酸铝钾对胎鼠肢芽细胞增殖和分化的影响

Influence of aluminum potassium sulfate on proliferation and differentiation of mouse embryo limb bud cells

目的研究硫酸铝钾对胎鼠肢芽细胞的增殖和分化的影响。方法应用微团培养法探讨不同浓度铝（0～100μmoL／L）对体外培养的小鼠胚胎肢芽细胞增殖和分化的影响。结果铝对肢芽细胞的50％增殖抑制浓度（IP50）和50％分化抑制浓度（ID50）分别为45．97和3．60μmol／L，IP50／ID50值为12．8，并有明显的剂量-反应关系。结论硫酸铝钾对胎鼠肢芽细胞的增殖和分化可能有抑制作用。

Objective To investigate the effects of KAl（ SO4 ） 2 on proliferation and differentiation of mouse embryonic limb bud cells. Methods With the technic of mieromass culture in vitro, the effects of KAI（ SO4 ） 2 （0 - 100 μmol/L） on the chondrogenesis of mouse embryo limb bud ceils was assessed. Results The concentrations of 50% inhibition dose of irradiation for cell proliferation （IP50） and the dose giving 50% of maximal inhibition （ID50） of KAl（ SO4 ）2 were ,14.94 μmol/L and 3.60 μmol/L respectively, and the ratio of IP50 and ID50 was 12. 8. There was an obvious dose-dependent response observed. Conclusion One of the teratogenie mechanisms of KAl（ SO4 ） 2 could be the inhibition of proliferation and differentiation of mouse embryo limb bud cells.