光敏核不育水稻61 kD特异性蛋白质的纯化和N-端序列分析

ANALYSIS ON AMINO-TERMINAL SEQUENCE AND PURIFICATION OF A 61 kD PROTEIN FROM PHOTOPERIOD SENSITIVE GENIC MALE-STERILE RICE

用制备型聚丙烯酰胺凝胶电泳和制备型等电聚焦纯化了曾报道的光敏核不育水稻 (Oryza sativa)农垦 58S叶绿体的特异性蛋白质 P2 ,得到 SDS- PAGE和等电聚焦 (IEF )纯的 P2。经 SDS- PAGE和 IEF测定 ,该纯蛋白质的分子量是 61 k D,等电点是 5.8。现称 P2为 P61。氨基酸序列分析表明 P61的 N-端氨基酸序列与水稻和大麦叶绿体 ATPaseβ亚基的 N-端氨基酸序列同源。

The specific protein P2, one of the three specific proteins (P1,P2 and P3) in chloroplasts from photoperiod sensitive genic male sterile rice previously reported was purified through preparative two dimensional gel electrophoresis and preparative isoelectric focusing (IEF) and from which an uniform P2, checked with SDS PAGE and IEF,was obtained.The molecular weight and isoelectric point was 61 kD and 5.8,respectively.Therefore,P2 was referred as P61. A search in databases revealed that the amino terminal sequence of P61 was identical to that of β subunit of chloroplast ATPase from barley and rice.