生长素极性运输的抑制对叶生长发育模式的影响

INHIBITION OF AUXIN POLAR TRANSPORT AFFECTING THE MODEL OF LEAF GROWTH AND DEVELOPMENT

以烟草 ( Nicotiana tabacum L.品种“革新一号”)无菌幼苗叶片为材料 ,在 MS培养基中分别加不同浓度的生长素极性运输抑制剂 (三碘苯甲酸、反式肉桂酸、9-羟基芴 - 9-羧酸 )和 2 mg/ L BA ,探讨不定芽分化的情况 ,在这些培养基上都观察到不同形态的喇叭状叶片形成。结果表明 ,再生喇叭叶的发生频率与培养基中抑制剂一定范围内的浓度密切有关。当三碘苯甲酸浓度为 7.5 mg/ L 时 ,喇叭叶的发生频率最高可达到 82 .1 % ,在再生不定芽的不同位置均观察到有喇叭叶的发生。实验证明 ,抑制生长素的极性运输可导致叶形态发生改变 。

Tobacco (Nicotiana tabacum L. cv.Gexin No.1) leaf slices were cultured in MS medium with different concentrations of auxin polar transport inhibitors (2,3,5 triiodobenzoic acid (TIBA), trans cinnamic acid (CA),and 9 hydooxyflurence 9 carboxylic acid (HFCA)) and their effects on bud formation were observed. Although the effective concentrations vary with different inhibitors, all of them induced the formation of trumpet shaped leaves. The frequencies of trumpet shaped leaves were increased with the concentrations of inhibitors in media, and it was up to 82.1% when cultured in the medium containing 7.5 mg/L TIBA. The trumpet shaped leaves were formed in different sites of the adventitious buds.These results indicated that inhibition of auxin polar transport could affect the morphogenesis of leaves, so the polar transport of auxin is essential for the bilateral symmetry of leaf growth.