BioStarM-140s芯片基因信息查询系统的构建及应用

Construction and application of searching system on BioStarM-140s microarray

应用生物信息学数据库查询的方法,对B ioS tarM-140s小鼠基因表达芯片上的14 112个基因进行相应注释信息的查询和所对应蛋白质功能的分类.首先参照人类综合型芯片上基因的功能分类,设定了14个蛋白质类别,然后采用V isual C++编程语言,构建了基因信息查询系统.应用该查询系统,进行了网络生物学数据库信息资源的自动直接查询,共搜索到有注释信息的基因12 070个;进一步按照所设定的14个蛋白质类别对12 070个基因进行分类查询,搜索到了与这14个分类相符合的、具有详细蛋白质功能注释信息的基因共1 606个.

In order to search annotated and functional information of 14112 genes on BioStarM-140s gene expression microarray, the bioinformatics method of database searching is used. First, 14 pro- tein function classes are decided referring to the gene classification of human integrative microarray. Then gene information searching system is constructed by using Visual C＋＋. According to the searching system constructed, 12 070 genes with annotated information are obtained by direct search- ing; in a further searching from 12070 genes, 1 606 genes with detail functional information and in terms of 14 classes designated are also found.