怀地黄85-5品种内遗传多样性的RAPD和ISSR分析

Genetic Diversity within Rehmannia Glutinosa Libosh.f. Hueichingensis (Chao et Schih) Hsiao Cultivar 85-5 Detected by RAPD and ISSR Markers

采用CTAB法提取了怀地黄85-5品种16个单株的基因组DNA,使用紫外分析和琼脂糖凝胶电泳检测其纯度和大小,利用RAPD及ISSR分析其品种内遗传多样性.从24个RAPD引物和43个ISSR引物中分别筛选出具有稳定多态性条带的RAPD引物3个和ISSR引物2个,分别对16个单株基因组DNA进行PCR扩增.3个RAPD引物共扩增出7条带,其中多态性条带为4个,多态性比率为57.14%.2个ISSR引物共扩增出17条带,其中多态性条带为11个,多态性比率为64.7%.结果表明,怀地黄85-5品种内存在遗传差异,但遗传多样性比地黄品种间的低.

The genomic DNAs were extracted by CTAB method from the young leaves of 16 individuals of Rehmannia Glutinosa Libosh. f. Hueichingensis （Chao et Schih） Hsiao cultivar 85-5 , whose purities and sizes were tested by UV method and agrose gel electrophoresis, respectively. Genetic diversity within cultivar 85-5 was assessed by RAPD and ISSR Markers. Three RAPD primers and two ISSR primers were selected from twenty-four RAPD ones and from forty-three ISSR ones to generate DNA fingerprints of these 16 individuals. Seven fragments were amplified with the three RAPD primers, four of which were polymorphic. Polymorphic percentage was 57.14%. Seventeen fragments were amplified with the two ISSR primers, eleven of which were polymorphic. Polymorphic percentage was 64.7%. The results showed that genetic difference occurred within cultivar 85-5, but genetic diversity was lower than that among different Rehmannia Glutinosa Libosh cultivars.