原发性胆汁性肝硬化抗线粒体抗体M2亚型的诊断价值

The clincal presentation of primary biliary cirrhosis and the diagnostic value of anti-mitochondrial antibody subtype

目的探讨抗线粒体亚型(AMA亚型)对原发性胆汁性肝硬化(PBC)的诊断价值。方法应用酶免疫斑点法检测血清中AMA-M2、M4、M9亚型。13例经临床诊断为PBC患者,44例非PBC肝胆病患者。结果13例PBC患者M2均阳性、M4及M9均阴性;44例非PBC肝胆病患者M2、M4、M9均阴性。5例PBC者肝活检4例为Ⅱ期、1例为Ⅳ期。结论血清AMA-M2抗体检测可作为临床诊断PBC的重要血清免疫学指标。

Objective To explore the diagnostic value of anti-mitochondfial antibody subtype（AMA-M2, M4 and M9） in the patients of primary biliary cirrhosis（PBC）. Methods The enzyme immune blot assay were used for the detection of AMA-M2, M4 and M9 antibodies in 13 PBC patients diagnosed by clinical persentation and 44 patients with other hepato-biliary diseases as control. Results All the 13 PBC patients diagnosed by clinical presentation were found to be M2 antibody positive while the M4 and M9 were all negative. The 44 patients with other hepato-biliary diseases were all negative in AMAM2, M4 and M9 antibedies. In the 5 of the 6 PBC patients who had received liver biopsy,4 patients were found to be in stage I1 of PBC and 1 patient in stage IV. Conclusion AMA-M2 antibody detection is an important and sencetive sereimmunological marker for the diagnosis of PBC.