肝细胞肝癌谷胱甘肽硫转移酶P1甲基化研究

Methylation of glutathione S-transferases P1 in hepatocellular carcinoma

目的本研究探讨谷胱甘肽硫转移酶（GST）PI启动子CpG岛甲基化与肝细胞肝癌（HCC）的关系。方法用甲基化特异性PCR技术检测53例HCC肿瘤组织及其癌旁非肿瘤组织中，GSTP1基因启动子CpG岛甲基化状况。结果GSTP1基因在HCC肿瘤组织中的甲基化率显著高于癌旁非肿瘤组织（X^2=19．08，P〈0．001），在Ⅲ-Ⅳ期肿瘤中的甲基化率显著高于Ⅰ-Ⅱ期肿瘤（X^2=4．84，P=0．028），在不同大小的肿瘤之间、单个结节与多个结节的肿瘤之间以及包膜完整的肿瘤与包膜不完整的肿瘤之间甲基化率的差异均无显著性。结论GSTP1启动子CDG岛甲基化可能参与肝细胞的痛性转变。

Objective To study the relationship between methylation of CpG islands of glutathione S-transferases （GST） P1 and hepatocellular carcinoma （HCC）. Methods Using methylation - special PCR, we studied the methylated status of CpG islands for GSTP1 gene in tumor tissues and its corresponding nontumor tissues of 53 HCC patients. Results The frequency of methylation of CpG islands for GSTP1 gene was significantly higher among the HCC tumor tissues when compared to the corresponding nontumor tissues （X^2 = 19.08, P 〈 0.001）, and significantly higher in stage Ⅲ-Ⅳ cases when compared to the stage Ⅰ-Ⅱ cases （X^2 = 4.84, P = 0.028）. All the differences of the frequency of methylation of CpG islands for GSTP1 gene between large tumors （diameter 〉 5 cm）and small tumors（ diameter ≤ 5 cm）, between tumors with single nodule and multiple nodules, and between well-encapsulated tumors and poor- encapsulated tumors were not statistical significant. Conclusion Methylation of CpG islands for GSTP1 gene maybe involved in the pathogenesis of HCC.