随机扩增杂交微卫星方法在大仓鼠种群遗传多态性研究中的应用

Application of Random Amplified Hybridization Microsatellite Markers in Polymorphic Analysis of the Greater Long-tailed Hamster Populations

随机扩增杂交微卫星(Random amplified hybridization microsatellites,RAHM)是一种复合RAPD扩增和寡核苷酸扫描的方法。该方法能够从RAPD产物凝胶上获得更多的信息,具有分析方法快速,高敏感性,能检测到高水平的多态性等优点。RAHM方法通过对RAPD扩增的DNA片段进行微卫星杂交来替代限制性内切酶对基因组DNA的消化,有助于揭示微卫星基因组克隆,进行微卫星引物的筛选。本文采用随机扩增杂交微卫星方法来检测小型哺乳动物大仓鼠(Techerskia triton)种群的遗传多态性,结果表明RAHM方法能够检测到大仓鼠种群中较高的多态性以及种群间的差异,这些条带模式可能代表真核基因组中另一种多态性标记的来源,可用于检测小型哺乳动物种群的遗传多态性。

Random amplified hybridisation microsatellites （RAHM）, a new system which combines RAPD amplification and oligonucleotide screening, can provide more information from analysis on RAPD gels, and it has several advantages including rapid analysis and high sensitivity for detecting high-level variability. RAHM is useful for revealing microsatellite genomic clones and selecting the microsatellite primers by substituting restrictions enzyme digestion of genomic DNA with microsatellite hybridization. Our study based on RAHM analysis revealed that this new technique could discern high level of polymorphism within and among populations of the Greater Long-tailed Hamster （ Tscherskia triton）. The result suggests that such banding patterns might represent an additional source of polymorphic markers in eukaryotes and could be used for detecting the genetic diversity of the small mammals populations.