人黑素瘤细胞A375 MIA/CD-RAP基因启动子的克隆及其鉴定

Cloning of human MIA/CD-RAP promoter and its identification

目的:克隆MIA/CD-RAP的启动子序列并对其进行酶切和DNA测序鉴定。方法:从培养的人黑素瘤细胞A375基因组DNA中扩增出MIA/CD-RAP启动子序列,并将其克隆到载体pGL2-Ba-sic上,再经酶切及PCR扩增鉴定和测序。结果:酶切电泳和DNA测序结果表明,已成功克隆了人黑素瘤MIA/CD-RAP启动子序列。结论:人黑素瘤MIA/CD-RAP启动子序列的克隆,可为进一步研究MIA/CD-RAP基因的表达调控奠定基础。

Objective： To clone and identify the MIA/CD- RAP promoter sequence in melanoma. Methods： MIA/CD - RAP promoter of human melanoma was amplified by PCR and cloned into vector pGL2 - Basic, which was cut out by restriction enzymes, and sequenced. Results： The recombinant plasmid of human MIA/CD- RAP promoter was successfully constructed and identified by PCR and DNA sequencing. Conclusion： Cloning of human MIA/CD- RAP promoter can form a base for the investigation of expression regulation of MIA/CD- RAP in melanoma in future.