甲胺磷对拟环纹豹蛛中肠蛋白消化酶活性影响的压电传感监测

Effect of methamidophos on midgut proteinase activity in the wolf spider, Pardosa pseudoannulata (Araneae: Lycosidae) assayed with piezoelectric bulk acoustic wave impedance analysis method

利用压电体声波阻抗分析法实时动态监测了拟环纹豹蛛Pardosapseudoannulata中肠蛋白消化酶对酪蛋白的酶促水解过程及不同浓度梯度(0.008%、0.016%、0.024%和0.032%)的甲胺磷农药对酶活性的影响,并用紫外分光光度法进行了验证。结果表明,合适低剂量(0.008%)的甲胺磷农药可显著地增强拟环纹豹蛛蛋白消化酶的活性,较高剂量(0.032%)的农药却显著抑制蛋白消化酶的活性,且水解过程稳态频移值可准确地反映酶活性与农药浓度的关系。本工作为研究低剂量农药增强蜘蛛控虫力的机理及酶活性的快速检测提供了一种可行的新方法。

The piezoelectric bulk acoustic wave （BAW） impedance analysis method was employed to monitor in situ the proteinase-catalyzed hydrolyzation of casein and the effect of methamidophos pesticide on the midgnt proteinase activity of the wolf spider, Pardosa pseudoanmdata, and its results were verified by ultraviolet spectroscopy. The results showed that hydrolyzation was significantly increased in the presence of low-dose methamidophos, whereas the hydrolyzation was significantly inhibited in the presence of high-dose methamidophos, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of methamidophos. The present work may have presented a novel method feasible for studying the mechanism of excitability of spiders under low-dose methamidophos pesticide and quick detection of proteinase activity.