噪声应激大鼠胃壁细胞形态和泌酸功能的变化

Changes of gastric parietal cell configuration and acid secretion under noise stress in rats

目的:建立白噪声致大鼠应激性溃疡模型(noise stress,NS),并研究噪声应激大鼠胃壁细胞形态和泌酸功能的变化。方法:采用白噪声应激建立NS大鼠模型。大鼠随机分为对照组、NS 4 h组及NS 8 h组,测定胃液pH值、胃黏膜溃疡指数(ulcer index,UI),用免疫荧光技术标记H+,K+-ATP酶,在激光共聚焦扫描显微镜下观察壁细胞形态并在荧光显微镜下分类、计数。结果:(1)NS 4 h组胃液pH值为2.22±0.05,显著低于对照组的2.59±0.21(P<0.05),NS 8 h组胃液pH值为1.31±0.06,与对照组和NS 4 h组相比均有显著差异(P<0.01)。(2)NS 4 h组UI为10.5±1.29,显著高于对照组(P<0.01),NS 8 h组UI达34.36±2.22,显著高于对照组(P<0.01)及NS 4 h组(P<0.05)。(3)NS 4 h组分泌相壁细胞数(55.5±3.42)/100,NS 8 h组为(62.0±3.46)/100,均显著高于对照组的(42.5±3.70)/100(P<0.01),其中NS 8 h组也显著高于NS 4 h组(P<0.05)。(4)胃液pH值与胃UI呈负相关(r=-0.85,P<0.05),与分泌相壁细胞数呈负相关(r=-0.89,P<0.05)。结论:白噪声应激可以成功的建立大鼠应激性溃疡模型;噪声应激大鼠胃酸分泌增加,胃酸在溃疡形成过程中起不可忽视的作用。

Objective：To establish a white noise-induced stress ulcer model in rats and to assess the changes of gastric parietal cell configuration and acid secretion under noise stress. Methods： Rats were randomly divided into 3 groups： NS 4 h,8 h and control groups. The rat NS model was induced by white noise. The pH values of gastric juice were measured by the pH/mV pH meter and the gastric ulcer index （UI） was evaluated. The stomach tissue was processed for immunofluorescence detection and the 13 subunit of H^＋ , K^＋-ATPase was immunostained. The gastric parietal cell configuration was observed with conventional microscopy and confocal laser scanning microscope. Results.. （1） The pH value of NS 4 h group （2.22 ± 0.05） was significantly lower than that of control group （2.59±0.21, P〈0.05） , but higher than that of NS 8 h group （1.31±0.06,P〈0.01）. （2） The UI of NS 4 h group （10.5±1.29） was significantly higher than that of the control group （P〈0.05）, but significantly lower than that of NS 8 h group （34. 36±2. 22, P〈0. 01）. （3） The reticular pattern parietal cells in NS 4 h group （55.5±3.42）/100 was significantly higher than that in control group （42.5±3.70/100, P〈0.01）, but lower than that in NS 8 h groups （62.0±3.46 /100,P〈0.05）. （4） The pH was negatively related with both UI （r=-0.85, P〈0.05） and the number of reticular parietal cells （r= -0.89, P〈0.05）. Conclusion： A white noise-induced stress ulcer model has been successfully established in rats. NS rats develop gastric mucosal lesions with increased acid secretion, which is closely related with the oxyntic function of parietal cells.