糖皮质激素抑制人前列腺癌PC-3细胞系增殖的可能机制

Inhibitory effects of dexamethasone on growth of human prostate cancer cell line PC-3:the possible mechanism

目的:观察地塞米松对前列腺癌PC-3细胞中转录因子NF-κB及其下游靶基因cyclinD1的调节作用,探讨其抑制PC-3细胞增殖的可能机制。方法:MTT法检测地塞米松对PC-3细胞增殖的影响;转染和报告基因的方法比较多种肿瘤细胞中NF-κB的基础转录活性并检测地塞米松对PC-3细胞NF-κB转录活性的调节;Western印迹法检测地塞米松对PC-3细胞cyclinD1蛋白表达的影响。结果:地塞米松抑制PC-3细胞的增殖;PC-3细胞中NF-κB处于组成型激活状态,地塞米松抑制PC-3细胞中转录因子NF-κB的转录活性;地塞米松下调PC-3细胞中cyclinD1蛋白的表达。结论:地塞米松抑制前列腺癌PC-3细胞的增殖,这可能与其抑制转录因子NF-κB的转录活性及其下游的靶基因cyclinD1的蛋白表达有关。

Objective：To investigate the regulatory effect of dexamethasone on NF-κB and its target gene cyclinD1 in human prostate cancer cell line PC-3, so as to explore the anti-proliferation mechanism of dexamethasone. Methods.. The inhibitory effects of dexamethasone on growth of PC-3 cells were determined by MTT. The basic transcriptional activity of NF-κB in many kinds of tumor cells and the effects of dexamethasone on the activation of PC-3 cell NF-κB were determined by reporter gene assay. The expression of cyclinD1 protein was determined by Western blots. Results： Dexamethasone inhibited the growth of PC- 3 cells and NF-κB was constitutively activated in the PC-3 cells. Dexamethasone also inhibited the activation of NF-κB and down-regulated the expression of cyclinD1 protein in PC-3 cells. Conclusion： Dexamethasone can inhibit cell growth of prostate cancer cell line PC-3 ,which might be related to the inhibitory effect of dexamethasone on the transcriptional activation of NF-κB and cyclinD1 protein.