福建省鼠形动物巴尔通体感染调查

The investigation on the infection of Bartonella species in rodent hosts in Fujian

目的了解福建省巴尔通体在鼠形动物中的感染分布状况。方法被检标本于2005年5～9月收集自福建省厦门市所捕捉活鼠，用5％兔心血的脑心浸液琼脂培养基分离巴尔通体，可疑菌落分纯1～4代；以聚合酶链反应（PCR）扩增特异性高的构檬酸合酶基因（gltA）的379bp片段，以证实为巴尔通体。结果共捕鼠形动物151只，分离到22株巴尔通体，分离率为14．57％。菌株分布于2属2目，其中褐家鼠8．97％，臭朐腈20．55％，臭朐腈带菌的报告在国内外尚属首次。结论本文报道首次证实巴尔通体在福建省鼠形动物中流行，且感染率高，需对感染分布状况开展进一步的流行病学调查；各种巴尔通体对应于不同的人类疾病，还需采用分子生物学方法作巴尔通体系统发育关系分析。臭朐腈在南方占室内鼠形动物的50％左右，组成比例有增大趋势，臭朐腈感染巴尔通体的流行病学意义不言而喻。

To study the infection status of Bartonella spp. in Fujian province. The blood sample of rat were collected from Xiamen,Fujian. Isolation of Bartonella was processed on 5% brain heart infusion agar media supplemented with rabbit blood. Bartonella-like isolate were confirmed by Polymerase Chain Reaction （PCR） amplification of Bartonella specific primers of the citrate syithase （gltA） gene. The 379bp fragment of gltA gene was sequenced. Twenty-two strains of Bartonella isolates were obtained from 151 blood samples collected and the infection rate was ahout 14.57 % ,the strains of Bartonella belonging to 2 genus of 2 orders,in which the infection rate of Sever rat was about 8.97% and Suncus murinus was about 20.55%. Aslo Suncus murinus with Bartonella was reported for the first time at home and abroad. In this paper it was indicated that the prevalence of Bartonella spp. was high in the most prevalent species of rodent hosts in Fujian. The finding of Bartonella in rodent hosts suggested the need for investigating the role of these agents as the source of human illness by the method of molecular biology. In the south of China,more than 50 percent of rodent hosts is made up by Suncus murines, so spoke for itself the epidemiologic significant of Suncus murinus which infect Bartonella.