Study of Multi-directional Derivation of Cord Blood Mononuclear Cells and Observation of Killing Activity to MGC-803 Gastric Cancer Cell Strain In Vitro

客观：象杀死活动到胃的癌症房间火车在试管内的变化一样学习脐带血 mononuclearcells 的多方向性的推导到 CD_3AK， LAK 和 CIK 房间。方法：CD_3mAb 和 IL-2 被用来导致 CD_3AK 房间，并且 IL-2 被用来导致 LAK 房间；IFN- γ在开始被使用，然后， IL-1， CD_3mAb 和 IL-2 为观察扩大并且分析他们的关系在 24 h 以后习惯于 induceCIK 房间。有教养的 CIK 房间的显型被流动血细胞计数分析。由使用胃的癌症房间作为目标房间拉紧的 MGC-803，随后， CD_3AK， LAK 和 CIK 房间的杀死的活动被使用 MTT 方法评估。结果：CD_3AK 和 CIK 房间的扩大活动都比 LAK 房间高远(P 【 0.05 ) 。扩大活动没在前期有 CIK 房间和 CD_3AK 房间之间的明显的差别，但是那比在大约 20thday 的 CD_3AK 房间在 CIK 房间更高是远的(P 【 0.05 ) 。流动血细胞计数表明 CD_3^+CD_(56 )^+ 房间的数量，在是有教养的 CIK 房间以后的主要受动器房间显著地被增加(P 【 0.05 ) ，而且， CD_8^+ 房间的数量显著地也被增加(P 【 0.05 ) 。到 MGC-803 胃的癌症房间紧张的杀死的活动 ofCD_3AK 和 CIK 房间都是显著地更高的 thanLAK 房间，当 CIK 房间的杀死的活动比 CD_3AK 房间强壮时(P 【 0.05 ).Conclusion：CIK 房间举办更强壮的扩大活动和杀死的活动，并且当更有效的杀死房间把采纳免疫疗法用于肿瘤，能被拿。

Objective： To study multi-directional derivation of cord blood mononuclear cells to CD3AK, LAK and CIK cells as well as changes of killing activity to gastric cancer cell strain in vitro. Methods： CD3mAb and IL-2 were used to induce CD3AK cells, and IL-2 was used to induce LAK cells; IFN-γ was used in the beginning, then IL-1, CD3mAb and IL-2 were used to induce CIK cells after 24 h for observing amplification and analyzing their relationship. The phenotypes of the cultured CIK cells were analyzed by flow cytometry. Subsequently, by using MGC-803 gastric cancer cell strain as target cells, the killing activity of CD3AK, LAK and CIK cells was evaluated by using MTT method. Results： The amplification activity of CD3AK and CIK cells was all far higher than LAK cells （P〈0.05）. The amplification activity had no obvious difference between CIK cells and CD3AK cells at prophase, but that was far higher in CIK cells than CD3AK cells at about 20^th day （P〈0.05）. The flow cytometry revealed that the amount of CD3^＋ CD56^＋ cells, major effector cells after CIK cells being cultured was significantly increased （P〈0.05）, moreover, the amount of CD8^＋ cells was significantly increased as well （P〈0.05）. The killing activities of CD3AK and CIK cells to the MGC-803 gastric cancer cell strain were all significantly higher than LAK cells, while the killing activity of CIK cells was stronger than CD3AK cells （P〈0.05）. Conclusion： CIK cells have stronger amplification activity and killing activity, and can be taken as more effective killing cells applied to the tumor adoptive immunotherapy.