摘要
客观:调查船边交货,船边交货 ligand (FasL ) 和 CD80on 的表示老鼠的房间表面尖锐 myelomonocytic 白血病房间线 WEHI-3 和功能 ofFasL。方法:船边交货, FasL 和 CD80 的表示被 flowcytometry 在 WEHI-3 房间表面上检测。同时, FasL 的功能被胸腺嘧啶核甙(~ 3H-TdR ) 决定 Incorporation.Results:WEHI-3 房间表面上的 CD80 和船边交货的表示分别地是 5.06%± 0 .41% 和 6.75%± 2 .31%(n=5 ) ,并且 FasL 的表示直到 63.73%± 5 .23%(n=5 ) 。apoptotic 率 ofYAC-1 房间当时,分别地是 26%± 4 .5% , 35%± 3 .2% 和 43%± 2 .7%(n=5 ) WEHI-3 (受动器房间, E ) 并且 Fas^+ YAC-1 房间(目标房间, T ) 在 3:1, 10:1 和 30:1 .Conclusion 的比率是有教养的:WEHI-3 房间表示高 FasL,低船边交货和 CD80,并且能导致 Fas^+YAC-1 房间的 apoptosis。
Objective: To investigate the expression of Fas, Fas ligand (FasL) and CD80 on the cell surface of mouse acute myelomonocytic leukemia cell line WEHI-3 and the function of FasL. Methods: The expression of Fas, FasL and CD80 was detected on WEHI-3 cell surface by flow cytometry. Simultaneously the function of FasL was determined by Thymidine (^3H-TdR) Incorporation. Results: The expression of CD80 and Fas on WEHI-3 cell surface was 5.06%±0.41% and 6.75%±2.31% (n=5) respectively, and the expression of FasL was up to 63.73%±5.23% (n=5). The apoptotic rate of YAC-1 cells was 26%±4.5%, 35%±3.2% and 43%±2.7% (n=5) respectively when WEHI-3 (effector cell, E) and Fas^+ YAC-1 cells (target cell, T) were cultured in the ratio of 3:1, 10:1 and 30:1. Conclusion: WEHI-3 cells express high FasL, low Fas and CD80, and can induce apoptosis of Fas^+ YAC-1 cells.
基金
Supported by a grant from National Natural Sciences Foundation of China (30240022).