依达拉奉对帕金森病大鼠多巴胺转运体保护作用的研究

Study on protective effect of Edaravone on dopamine transporter in rat models of Parkinson's disease

目的探讨依达拉奉对帕金森病(PD)大鼠多巴胺转运体(DAT)的保护作用。方法PD模型组大鼠,分为依达拉奉大剂量(3.0mg/kg)组、中剂量(1.0mg/kg)组、小剂量(0.3mg/kg)组及生理盐水组(对照组),每天两次腹腔注射治疗两周,停药1周后,用γ放射性计数仪测定双侧纹状体、大脑皮质、小脑DAT含量,计算脑组织ID值。结果大剂量组右侧纹状体放射性计数(0.47±0.06)、中剂量组(0.37±0.02),与对照组(0.25±0.01)相比差异有显著性(均P<0.05),大、中、小剂量组和未治疗组左侧脑组织放射性计数与右侧相应部位比较差异均有显著性(均P<0.05)。结论大剂量依达拉奉可增强脑组织的抗氧化能力,以纹状体最为显著。

Objective To investigate the protective effect of Edaravone on dopamine transporter in rat models of Parkinson disease. Methods Rat models of Parkinson disease were induced by injection 6-OHDA into right medial forebrain bundle. Edaravone at different doses （3.0 mg/kg, 1.0 mg/kg or 0. 3 mg/kg） was injected intraperitoneally twice daily for two weeks. The same dose of normal saline was injected in the control group. One week after the treatment, the γ-radiation of rat bilateral striatum, cerebral cortex and cerebella cortex of each group was measured by a γ-counter and the brain tissue ID value was calculated. Results There was a significant difference of the radiation count in fight striatum between the large dose group （ 0. 47±0. 06 ） , medium doss group （0.37±0. 02 ） and the control group （0.25 ± 0.01 ）（P〈 0. 05）. The radiation counts in the right side of the brain were significantly different the left side in the all groups （ all P 〈 0. 05 ）. Condusion Edaravone may enhance antioxidation of brain tissues, especially in striatum.