三羟异黄酮对大鼠肠缺血再灌注损伤的保护作用

Protective effects of genistein on intestinal ischemia-reperfusion injury in rats

目的:研究三羟异黄酮对肠缺血再灌注损伤的影响。方法:24只雄性Wistar大鼠随机分为假手术组(S组)、肠缺血再灌注损伤组(IIRI组)和三羟异黄酮组(G组)。观察三组肠组织病理学变化,测定比较肠组织湿/干重比(W/D)、髓过氧化物酶(MPO)活性、细胞因子诱导的中性粒细胞趋化因子-1(CINC-1)含量和血浆D-乳酸含量。结果:与S组比较,IIRI组肠损伤严重,W/D、MPO活性、CINC-1含量和血浆D-乳酸含量升高(P<0.01);与IIRI组比较,G组肠损伤明显减轻,W/D、MPO活性、CINC-1含量和血浆D-乳酸含量降低(P<0.05或P<0.01)。结论:三羟异黄酮对肠缺血再灌注损伤具有保护作用,可能与其下调CINC-1的表达而抑制中性粒细胞在肠组织的聚集有关。

Objective：To investigate the effects of genistein on intestinal ischemia-reperfusion injury in rats. Methods：Twenty-four Wistar rats were randomly assigned to one of three groups： sham operation group （S group）, intestinal ischemia-reperfusion injury group （IIRI group） and genistein group （G group）. Wet-to-dry weight （W/D） ratio, myeloperoxidase （MPO） activity, cytokine-induced neutrophil chemoattractant-1 （CINC-1） content in intestinal tissues and plasma D-lactate level were measured. The histopathological changes in intestines were also assayed. Results：W/D, MPO activity, CINC- 1 content in intestinal tissues and plasma D-lactate level were significantly higher in IIRI group than that in S group （P〈0.01）. The values in these indexes were significantly lower in G group than that in IIRI group （P〈0.05 or P〈0.01）. Ischemia-reperfusion also induced extensive histepathological intestine injury, which was reduced by genistein pretreatment. Conclusion：Pretreatment with genistein has protective effect on intestinal ischemia-reperfusion injury in rats, which may involve in an inhibition of neutrophilic recruitment possibly through an inhibition of CINC-1 expression.