金雀异黄素对去卵巢大鼠脂蛋白(a)及脂类代谢的影响

The influence of Genistin on lipoprotein(a)and lipid metabolism in ovariectomized female rats

目的:研究金雀异黄素对去卵巢大鼠血液脂蛋白[Lp(a)]及脂类代谢的影响。方法:将雌性SD大鼠63只双侧摘除卵巢,手术后1w按血清胆固醇水平随机分为7组,分别进行高脂饲料、雌激素,低、中、高剂量干预及假手术处理,并设正常对照组,灌胃给药3个月,每周称重一次,实验结束后取血清、内脏及骨骼标本,测定胆固醇(TC),甘油三酯(TG)、血清高、低密度脂蛋白胆固醇(HDL、LDL-C)、Lp(a)及抗氧化等指标。结果:经金雀异黄素干预后大鼠体重、TG、LDL-C、Lp(a)、GSH-Px活力与对照组间存在差异;干预组LDL-C显著低于高脂组,干预对GSH-Px活性存在影响。结论:金雀异黄素对去卵巢大鼠体重、血清中TG、LDL-C、Lp(a)、GSH-Px存在一定的调节作用,对去势大鼠的高脂血症存在拮抗作用且能在一定程度上改善体内的脂质过氧化状态。

Objective： To study the effects of genistin in lipoprotein （a）[Lp （a）]and lipid metabolism in ovariectomized rats. Methods：63 female SD rats after ovariectomized for 1 week were randomly divided into 7 groups according to the cholesterol level, intervention with hyper-lipoids feeds, estrogen, low, middle, high dose intervention, sham operation, normal control groups was set up, intragastric administration with different dose of genistin for 3 months. Body weight of rats was weighted ad unam vicem per week and rats were killed, serum biochemical indexes, viscera and steleton were collected and cholesterol（TC）,triglyceride（TG）, high, low density lipoprotein（HDL, LDL-C）and Lp（a）were measured. Results：There were diferences amone rats body mass,TG, LDL-C,Lp （a） and GSH-Px vigour in intervented and control groups after intervention with genistin. The level lof genistin LDL-C in intervented groups was significantly lower than that in hyper-lipoids groups. There are persistence influence for GSH-Px vigour on account of intervention. Conclusion：It is concluded that genistin hay definite modulation on the rats body mass, biochemical indexes of TG, LDL-C, Lp （a）, GSH-Px in ovariectomized female rats,because of their weak estrogenic activity. Genistin has antagonistic action on hyperlipoidemia and improve the peroxidation state of lipid in ovariectomized rats.