高效液相色谱法测定人血浆中盐酸环丙沙星的方法学研究

Determination of ciprofloxacin hydrochloride in the human plasma by HPLC

目的 建立血浆中盐酸环丙沙星含量的RP—HPLC测定方法。方法 采用Diamonsil C18色谱柱（200mm×4．6mm，5μm）；乙腈-20mmol·L^-1磷酸氢二钾／磷酸二氢钾缓冲溶液（用磷酸调pH至2．83）=25：75为流动相，用前经0．45μm的滤膜过滤，超声脱气；流速为1．0mL·min^-1；荧光检测波长为295／440nm；柱温为35℃，进样量20μL。结果 此方法最低检测浓度为5ng·mL^-1，线性范围为10～4000ng·mL^-1，方法平均回收率在99．11％～103．80％，日内及日间精密度的相对标准差分别〈2％和5％。结论 该方法简单、快速，灵敏度高，重复性好。

OBJECTIVE To determine eiprofloxacin hydrochloride in the human plasma by reversed phase high performance liquid chromatography. METHODS Ciprofloxaein hydroehloride was successfully separated using a Diamonsil C18 column （200 mm×4.6mm,5μm）. The mobile phase consisted of aeetonitrile-20 mmol ·L^-1 dibasicpotassium phosphate and monobasicpotassium phosphate buffer solution （25 ： 75） （adjusted to pH 2.83 using phosphoric acid） and was degassed ultrasonically before use. Each component of the mobile phase was filtered through a 0. 45 μm membrane. The column temperature was 35 ℃ and the flow rate was 1.0 mL ·mL^-1. Injection volume was 20 μL. The peak of eiprofloxacin hydroehloride was monitored with fluorescence detector （290/440 nm）. RESULTS The detection limit was 5 ng ·mL^-1. The linear range was 10～4 000 ng ·mL^-1. The average recovery was 99.11%～103. 80%. RSD of intra-day and inter-day was less than 2% and 5%, respectively. CONCLUSIONS The method is simple, rapid, sensitive and producible.