高效液相色谱法测定苯酚滴耳液中苯酚的含量

HPLC determination of phenol in phenol ear drops

目的 采用高效液相色谱法（HPLC）测定苯酚的含量。方法 以XDB-C18为色谱柱，以甲醇-水（60：40）为流动相，检测波长为268nm，流速为1．0mL·min^-1，柱温为25℃。结果 苯酚在1．6～20μg线性关系良好，r=0．9991，平均回收率为99．4％，RSD为0．3％。结论 该质量标准的研究有效控制了苯酚滴耳液的质量，方法简单、结果准确、灵敏度高。

OBJECTIVE An HPLC method was developed to determine the content of phenol. METHODS It was determined on an XDB-C18 column, using methylalcohol-water （60 ： 40） as the mobile phase at a flow rate of 1.0 mL · min^-1 , and was detected at 268 nm. The column temperature was 25℃. RESULTS The linear relationship of the peak area was determined as 1.6～20μg by HPLC, with a regression coefficient of 0. 999 1. The average recovery rate was 99.4%, and RSD=0. 3%. CONCLUSIONS The method is simple, rapid and accurate. It can be used for the determination of phenol in ear drops.